A HIGH PERFORMANCE ELECTROPORATION CHIP INTEGRATING MULTI-WELL PLATE AND ANNULAR INTERDIGITAL MICROELECTRODES | |
Wei, Zewen ; Huang, Huang ; Liang, Zicai ; Li, Zhihong | |
2010 | |
关键词 | BIOMOLECULES CELLS |
英文摘要 | Electroporation, in which electric pulses are applied to create transient pores in the cell membrane, is widely used to introduce biologically active molecules into cells. Here we report a novel electroporation chip with great performance and good compatibility with the standard multi-well plate used in biological research. We demonstrated a prototype chip integrating with a 12-well plate and successfully electroporated both adherent and supernatant cells. Using the standard expression cell line HEK-293 and expression vector pEGFP-C3, we realized excellent cell viability (80%) and transfection rate (90%), both much higher than those in previously reported methods.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000278416400236&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Electrical & Electronic; Nanoscience & Nanotechnology; EI; CPCI-S(ISTP); 1 |
语种 | 英语 |
DOI标识 | 10.1109/MEMSYS.2010.5442365 |
内容类型 | 其他 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/293051] ![]() |
专题 | 信息科学技术学院 |
推荐引用方式 GB/T 7714 | Wei, Zewen,Huang, Huang,Liang, Zicai,et al. A HIGH PERFORMANCE ELECTROPORATION CHIP INTEGRATING MULTI-WELL PLATE AND ANNULAR INTERDIGITAL MICROELECTRODES. 2010-01-01. |
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