Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced   Graphene Oxide Field-Effect Transistor Biosensor

doi:10.1021/nn4063424

CORC > 北京大学 > 信息科学技术学院

	Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor
	Cai, Bingjie ; Wang, Shuting ; Huang, Le ; Ning, Yong ; Zhang, Zhiyong ; Zhang, Guo-Jun
刊名	acs nano
	2014
关键词	graphene field-effect transistor biosensor detection peptide nucleic acid DNA hybridization SILICON NANOWIRE BIOSENSOR FREE ELECTRICAL DETECTION GRAPHITE OXIDE LARGE-AREA PROTEIN FILMS NANOSHEETS SHEETS SENSORS BINDING
DOI	10.1021/nn4063424
英文摘要	A reduced graphene oxide (R-GO)-based field-effect transistor (FET) biosensor used for ultrasensitive label-free detection of DNA via peptide nucleic acid (PNA) DNA hybridization is reported. In this work, R-GO was prepared by reduction of GO with hydrazine, and the FET biosensor was fabricated by drop-casting the R-GO suspension onto the sensor surface. PNA instead of DNA as the capture probe was employed, and DNA detection was performed through PNA DNA hybridization by the R-GO FET biosensor. The detection limit as low as 100 fM was achieved, which is 1 order of magnitude lower than that of the previously reported graphene FET DNA biosensor based on DNA DNA hybridization. Moreover, the R-GO FET biosensor was able to distinguish the complementary DNA from one-base mismatched DNA and noncomplementary DNA. Interestingly, the fabricated DNA biosensor was found to have a regeneration capability. The developed R-GO FET DNA biosensor shows ultrasensitivity and high specificity, indicating its potential applications in disease diagnostics as a point-of-care tool.; Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; SCI(E); EI; 20; ARTICLE; zyzhang@pku.edu.cn; zhanggj@hbtcm.edu.cn; 3; 2632-2638; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/214989]
专题	信息科学技术学院
推荐引用方式 GB/T 7714	Cai, Bingjie,Wang, Shuting,Huang, Le,et al. Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor[J]. acs nano,2014.
APA	Cai, Bingjie,Wang, Shuting,Huang, Le,Ning, Yong,Zhang, Zhiyong,&Zhang, Guo-Jun.(2014).Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor.acs nano.
MLA	Cai, Bingjie,et al."Ultrasensitive Label-Free Detection of PNA-DNA Hybridization by Reduced Graphene Oxide Field-Effect Transistor Biosensor".acs nano (2014).