Degradation of MXC by host/guest-type immobilized laccase on magnetic tubular mesoporous silica

doi:10.1016/j.bej.2015.02.012

CORC > 北京大学 > 环境科学与工程学院

	Degradation of MXC by host/guest-type immobilized laccase on magnetic tubular mesoporous silica
	Yang, Yuxiang ; Wei, Qinmei ; Zhang, Jianbo ; Xi, Yanjie ; Yuan, Hongming ; Chen, Cheng ; Liu, Xiangnong
刊名	biochemical engineering journal
	2015
DOI	10.1016/j.bej.2015.02.012
英文摘要	In this paper, magnetic host/guest-type immobilized laccase was prepared by co-adsorption of super-paramagnetic particles (SPMNPs) and laccase into the pore channels of tubular mesoporous SiO2 by the 'size-matching effect'. After immobilization, the thermal stability and repetition usage-ratio of immobilized laccase were improved significantly, the loading amount of enzyme and SPMNPs was determined by Lowry's method and EDS (Energy Dispersive Spectroscopy), respectively. Under the optimal conditions, the removal efficiency of MXC (Methoxychlor) by immobilized laccase reached 69.4% and the removal efficiency still remained 45.4% after six cycles of operations. The studies on degradation kinetics of MXC by free and immobilized laccase were also carried out, respectively. In virtue of GC-MS, 1H NMR and 13C NMR analysis, (CH3OC6H5)2CCHCl and 1,1-diphenylethylene were identified as intermediate and final degradation products of MXC, respectively, further demonstrating the degradation mechanism of MXC by the immobilized laccase. ? 2015 Elsevier B.V.; SCI(E); EI; 0; ARTICLE; yxyang@ecust.edu.cn; 111-118; 97
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/263540]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Yang, Yuxiang,Wei, Qinmei,Zhang, Jianbo,et al. Degradation of MXC by host/guest-type immobilized laccase on magnetic tubular mesoporous silica[J]. biochemical engineering journal,2015.
APA	Yang, Yuxiang.,Wei, Qinmei.,Zhang, Jianbo.,Xi, Yanjie.,Yuan, Hongming.,...&Liu, Xiangnong.(2015).Degradation of MXC by host/guest-type immobilized laccase on magnetic tubular mesoporous silica.biochemical engineering journal.
MLA	Yang, Yuxiang,et al."Degradation of MXC by host/guest-type immobilized laccase on magnetic tubular mesoporous silica".biochemical engineering journal (2015).