Production of diosgenin from yellow ginger (Dioscorea zingiberensis C.   H. Wright) saponins by commercial cellulase

doi:10.1007/s11274-009-0285-y

CORC > 北京大学 > 环境科学与工程学院

	Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase
	Liu, Wei ; Huang, Wen ; Sun, WeiLing ; Zhu, YuLing ; Ni, JinRen
刊名	world journal of microbiology biotechnology
	2010
关键词	Diosgenin Steroidal saponins Cellulase Biotransformation STEROIDAL SAPONINS CURVULARIA-LUNATA PSEUDOJAPONICA YAMAMOTO SUBSTRATE-SPECIFICITY RHAMNOSIDASE ACTIVITY METHYL PROTODIOSCIN WASTE-WATER HYDROLYSIS TRANSFORMATION GLUCOAMYLASE
DOI	10.1007/s11274-009-0285-y
英文摘要	A commercial cellulase was first assessed to be effective in hydrolyzing glycosyl at the C-3 and C-26 positions in steroidal saponins from yellow ginger (Dioscorea zingiberensis C. H. Wright) to diosgenin, a very important chemical in the pharmaceutical industry. The effect of different parameters on enzyme hydrolysis was further investigated by systematically varying them. The highest yield was achieved when the hydrolysis ran at 55 degrees C and pH 5.0 with an enzyme to substrate ratio of 15 x 10(3) U/g. The biotransformed products identified using TLC and HPLC confirmed that the cellulase was capable of releasing diosgenin from steroidal saponins. Moreover, the biotransformation process was explored by LC-MS and LC-MS/ MS analysis. Enzymatic hydrolysis together with 40 % of the original sulphuric acid used increased the diosgenin yield by 15.4 +/- 2.7% than traditional method. Therefore, the commercial cellulase may serve as a promising tool for industrial diosgenin production and for further use in saponin modification.; Biotechnology & Applied Microbiology; SCI(E); EI; PubMed; 12; ARTICLE; 7; 1171-1180; 26
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158891]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Liu, Wei,Huang, Wen,Sun, WeiLing,et al. Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase[J]. world journal of microbiology biotechnology,2010.
APA	Liu, Wei,Huang, Wen,Sun, WeiLing,Zhu, YuLing,&Ni, JinRen.(2010).Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase.world journal of microbiology biotechnology.
MLA	Liu, Wei,et al."Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase".world journal of microbiology biotechnology (2010).