A novel beta-glucosidase from Aspergillus fumigates releases diosgenin   from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW)

doi:10.1007/s11274-011-0907-z

CORC > 北京大学 > 环境科学与工程学院

	A novel beta-glucosidase from Aspergillus fumigates releases diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW)
	Lei, Jing ; Niu, Hai ; Li, Tianhong ; Huang, Wen
刊名	world journal of microbiology biotechnology
	2012
关键词	Aspergillus fumigates beta-Glucosidase Thermostable Spirostanosides SAPONIN-RHAMNOSIDASE ACTIVITY STEROIDAL SAPONIN SUBSTRATE-SPECIFICITY CURVULARIA-LUNATA GLUCOAMYLASE PURIFICATION DIOSCIN
DOI	10.1007/s11274-011-0907-z
英文摘要	A beta-glucosidase effectively releasing diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW), named AfG, was purified from a strain of Aspergillus fumigates. The molecular weight of AfG was 113 kDa. Analysis of protein fragments by ESI-Q-TOF indicated that AfG was a beta-glucosidase. The circular dichroism spectrum suggested that the main secondary structure of AfG in Milli-Q water was alpha-helixes. Atomic force microscopy revealed that it was a globular protein. AfG maintained high activity from pH 3.6 to 5.0 and from 50 to 90A degrees C. With the strong heat stability, AfG retained 55% of its original activity at 65A degrees C for 120 h. AfG utilized muti-3-O-glycosides of various steroidal saponins from DZW as substrate, such as trillin, diosgenin diglucoside, dioscin, deltonin and gracillin, to yield diosgenin, suggesting the possibility of producing diosgenin from total saponins of DZW using a single enzyme.; Biotechnology & Applied Microbiology; SCI(E); EI; PubMed; 9; ARTICLE; 3; 1309-1314; 28
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158836]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Lei, Jing,Niu, Hai,Li, Tianhong,et al. A novel beta-glucosidase from Aspergillus fumigates releases diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW)[J]. world journal of microbiology biotechnology,2012.
APA	Lei, Jing,Niu, Hai,Li, Tianhong,&Huang, Wen.(2012).A novel beta-glucosidase from Aspergillus fumigates releases diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW).world journal of microbiology biotechnology.
MLA	Lei, Jing,et al."A novel beta-glucosidase from Aspergillus fumigates releases diosgenin from spirostanosides of Dioscorea zingiberensis C. H. Wright (DZW)".world journal of microbiology biotechnology (2012).