Degradation of 2,4,6-trinitrotoluene (TNT) by immobilized   microorganism-biological filter

doi:10.1016/j.procbio.2010.03.006

CORC > 北京大学 > 环境科学与工程学院

	Degradation of 2,4,6-trinitrotoluene (TNT) by immobilized microorganism-biological filter
	Wang, Zhongyou ; Ye, Zhengfang ; Zhang, Mohe ; Bai, Xue
刊名	process biochemistry
	2010
关键词	TNT Biodegradation Anaerobic degradation kinetic TNT metabolites Bacterial community GRADIENT GEL-ELECTROPHORESIS WASTE-WATER TREATMENT 16S RIBOSOMAL-RNA AERATED FILTER PSEUDOMONAS-PUTIDA ELECTRON-DONORS BIODEGRADATION REDUCTION PCR EXPLOSIVES
DOI	10.1016/j.procbio.2010.03.006
英文摘要	The combined process of immobilized microorganism-biological filter was used to degrade TNT in an aqueous solution. The results showed that the process could effectively degrade TNT, which was not detected in the effluent of the system. GC/MS analysis identified 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT), 4-amino-2,6-dinitrotoluene (4-A-2,6-DNT), 2,4-diamino-6-nitrotoluene (2,4-DA-6-NT) and 2,4-diamino-6-nitrotoluene (2,6-DA-4-NT) as the main anaerobic degradation products. In addition, the Haldane model successfully described the anaerobic degradation of TNT with high correlation coefficients (R(2) = 0.9803). As the electron donor, ethanol played a major role in the TNT biodegradation. More than twice the theoretical requirement of ethanol was necessary to achieve a high TNT degradation rate (above 97.5%). Moreover, Environment Scan Electron Microscope (ESEM) analysis revealed that a large number of globular microorganisms were successfully immobilized on the surface of the carrier. Further analysis by Polymerase Chain Reaction (PCR)-Denaturing Gradient Gel Electrophoresis (DGGE) demonstrated that the special bacterial for TNT degradation may have generated during the domestication with TNT for 150 clays. The dominant species for TNT degradation were identified by comparing gene sequences with Genebank. (C) 2010 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000277908100025&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical; SCI(E); EI; 33; ARTICLE; 6; 993-1001; 45
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/158490]
专题	环境科学与工程学院
推荐引用方式 GB/T 7714	Wang, Zhongyou,Ye, Zhengfang,Zhang, Mohe,et al. Degradation of 2,4,6-trinitrotoluene (TNT) by immobilized microorganism-biological filter[J]. process biochemistry,2010.
APA	Wang, Zhongyou,Ye, Zhengfang,Zhang, Mohe,&Bai, Xue.(2010).Degradation of 2,4,6-trinitrotoluene (TNT) by immobilized microorganism-biological filter.process biochemistry.
MLA	Wang, Zhongyou,et al."Degradation of 2,4,6-trinitrotoluene (TNT) by immobilized microorganism-biological filter".process biochemistry (2010).