荧光各向异性结合同步荧光法研究1-羟基芘与牛血清白蛋白的相互作用

doi:10.7503/cjcu20150119

CORC > 北京大学 > 城市与环境学院

	荧光各向异性结合同步荧光法研究1-羟基芘与牛血清白蛋白的相互作用; Interaction of 1-Hydroxypyrene with BSA Using Fluorescence Anisotropy and Synchronous Fluorescence Analysis Methods
	张静 ; 陈薇晓 ; 张唯 ; 段滢 ; 朱玉秀 ; 朱亚先 ; 张勇
刊名	高等学校化学学报
	2015
关键词	荧光各向异性 1-羟基芘牛血清白蛋白结合常数构型变化作用方式 Fluorescence anisotropy 1-Hydroxypyrene (1-OHP) Bovine serum albumin (BSA) Binding constant Conformation change Interaction mode HUMAN SERUM-ALBUMIN POLYCYCLIC AROMATIC-HYDROCARBONS SUBDOMAIN IB PROTEIN BOVINE BINDING SPECTROSCOPY AGGREGATION DRUGS LIGHT
DOI	10.7503/cjcu20150119
英文摘要	在模拟生理条件下,应用荧光各向异性法研究了多环芳烃(PAHs)代谢标志物1-羟基芘(1-OHP)与牛血清白蛋白(BSA)的相互作用,并结合同步荧光法研究作用过程中BSA的构型变化,初步探讨了二者的结合方式.研究结果表明,1-OHP与BSA有较强的结合作用,形成1∶1复合物,平均结合平衡常数为3.63×106L/mol,且其结合作用强弱随着BSA浓度大小发生变化.1-OHP可与BSA的色氨酸残基结合,使BSA构型发生变化,进而使色氨酸残基周围环境的疏水性降低.; Fluorescence anisotropy was employed to investigate the interaction of 1-hydroxypyrene(1-OHP), the metabolism biomarker of PAHs in vivo, with a model transport protein of bovine serum albumin (BSA) under simulated physiological conditions. Combined with synchronous fluorescence spectra, the conformation transition of BSA was also investigated. The experiment results showed that 1-OHP can bind to BSA strongly and a 1:1 complex was formed, with an average binding equilibrium constant of 3. 63 x10(6) L/mol. Also as the amounts of BSA differ, the interaction of 1-OHP and BSA was dominated by strong and weak binding modes alternately. Moreover, 1-OHP can bind to tryptophan residues and induce the conformational and micro-environmental changes of BSA, increasing the tryptophan residue of BSA exposuring to a less hydrophobic micro-environment.; the National Natural Science Foundation of China; the Fundamental Research Funds for the Central Universities,China; the National Training Program of Innovation and Enterpreneurship for Undergraduates,China (No.DC2013035).国家自然科学基金; 中央高校基本科研业务费专项资金; 国家级大学生创新创业项目基金; SCI(E); EI; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); yzhang@xmu.edu.cn; 8; 1511-1516; 36
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417240]
专题	城市与环境学院
推荐引用方式 GB/T 7714	张静,陈薇晓,张唯,等. 荧光各向异性结合同步荧光法研究1-羟基芘与牛血清白蛋白的相互作用, Interaction of 1-Hydroxypyrene with BSA Using Fluorescence Anisotropy and Synchronous Fluorescence Analysis Methods[J]. 高等学校化学学报,2015.
APA	张静.,陈薇晓.,张唯.,段滢.,朱玉秀.,...&张勇.(2015).荧光各向异性结合同步荧光法研究1-羟基芘与牛血清白蛋白的相互作用.高等学校化学学报.
MLA	张静,et al."荧光各向异性结合同步荧光法研究1-羟基芘与牛血清白蛋白的相互作用".高等学校化学学报 (2015).