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	Inhibitory kinetics of citric acid on beta-N-acetyl-D-glucosaminidase from prawn (Litopenaeus vannamei)
	Xie, Xiao-Lan ; Hu, Yong-Hua ; Wang, Ling-Ling ; Chen, Chao-Qi ; Huang, Qian-Sheng ; Zhou, Han-Tao ; Chen, Qing-Xi
	2010
关键词	Litopenaeus vannamei beta-N-acetyl-D-glucosaminidase Citric acid Inhibition Kinetics SUPPRESSION SUBTRACTIVE HYBRIDIZATION PENAEUS-VANNAMEI ENZYME-ACTIVITY HEPATOPANCREAS HEXOSAMINIDASE PURIFICATION CHITOBIASE
DOI	10.1016/j.fsi.2010.07.004
英文摘要	NAGase (EC.3.2.1.52) from crustaceans has the important roles in immunity, molting and digestion of chitinous foods. In this paper, the effects of citric acid on the activity of NAGase from Litopenaeus vannamei for the hydrolysis of pNP-NAG have been studied. The results showed that appropriate concentrations of citric acid could lead to reversible inhibition on NAGase and IC(50) was estimated to be 5.00 +/- 0.35 mM. Using the plots of Lineweaver-Burk, the inhibition of NAGase by citric acid belongs to competitive type, the inhibitory equilibrium constant for citric acid binding with free NAGase, K([), is 3.26 +/- 0.25 mM. The inhibitory kinetics of citric acid on NAGase in the appropriate concentrations of citric acid has been studied using the kinetic method of substrate reaction. The time course of NAGase for the hydrolysis of pNP-NAG in the presence of different concentrations of citric acid showed that at each citric acid concentration, the rate decreased with increasing time until a straight line was approached. The results show that the inhibition of NAGase by citric acid is a slow, reversible reaction with fractional remaining activity. The microscopic rate constants are determined for the reaction on citric acid with NAGase. (C) 2010 Elsevier Ltd. All rights reserved.; Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences; SCI(E); 2; ARTICLE; 4; 674-678; 29
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/242878]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Xie, Xiao-Lan,Hu, Yong-Hua,Wang, Ling-Ling,et al. Inhibitory kinetics of citric acid on beta-N-acetyl-D-glucosaminidase from prawn (Litopenaeus vannamei)[J],2010.
APA	Xie, Xiao-Lan.,Hu, Yong-Hua.,Wang, Ling-Ling.,Chen, Chao-Qi.,Huang, Qian-Sheng.,...&Chen, Qing-Xi.(2010).Inhibitory kinetics of citric acid on beta-N-acetyl-D-glucosaminidase from prawn (Litopenaeus vannamei)..
MLA	Xie, Xiao-Lan,et al."Inhibitory kinetics of citric acid on beta-N-acetyl-D-glucosaminidase from prawn (Litopenaeus vannamei)".(2010).

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