Characterization of Pseudomonas mendocina LR capable of removing   nitrogen from various nitrogen-contaminated water samples when   cultivated with Cyperus alternifolius L.

doi:10.1016/j.jbiosc.2012.03.008

CORC > 北京大学 > 城市与环境学院

	Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L.
	Feng, Zhihang ; Li, Xinghui ; Lu, Chenchen ; Shen, Zhenguo ; Xu, Fuliu ; Chen, Yahua
刊名	journal of bioscience and bioengineering
	2012
关键词	Denitrification Pseudomonas mendocina Root exudates Plant-microbe interactions Bioaugmentation AEROBIC DENITRIFYING BACTERIA MICROBIAL COMMUNITY STRUCTURE WASTE-WATER HEAVY-METALS NITRATE POLLUTION DENITRIFICATION SOIL PLANTS GROUNDWATER CARBON
DOI	10.1016/j.jbiosc.2012.03.008
英文摘要	A new strain of denitrifying bacterium, Pseudomonas mendocina LR, was isolated from rhizosphere soil of aquatic plants living in a river contaminated with industrial wastewater and domestic sewage. The isolate was found to fully remove as much as 613.2 mg nitrate in 60 h under stationary culture conditions. The effects of carbon sources and nitrogen sources on nitrogen removal were investigated using a modified denitrification medium (DM). Sodium citrate was identified as the most effective source of carbon. The ability of LR to adapt to different nitrogen sources, including nitrite, indicated that LR could be used in the purification of wastewater containing different forms of nitrogen. The optimal C/N ratio was 7 for LR, and it was resistant to antibiotics Amp, Chl, Ery, and Str. Plant-microbe bioaugmentation was performed to remove nitrogen dissolved in Hoagland medium and natural wastewater. An increased rate of nitrogen removal was observed when root exudates of Cyperus alternifolius L. were added simultaneously with LR. LR was not able to survive in the natural wastewater unless root exudates from umbrella grass were added. LR cultured with umbrella grass exhibited a maximal nitrogen reduction rate of 95.9% and 97.3% in Hoagland medium and wastewater, respectively. This shows that bioaugmentation utilizing plant-microbe interactions can be an effective and exhaustive means of removing nitrogen and may be an attractive approach to nitrogen reduction in natural environments and wastewater treatment factories. (c) 2012, The Society for Biotechnology, Japan. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000308519400011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biotechnology & Applied Microbiology; Food Science & Technology; SCI(E); EI; 5; ARTICLE; 2; 182-187; 114
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/231975]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Feng, Zhihang,Li, Xinghui,Lu, Chenchen,et al. Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L.[J]. journal of bioscience and bioengineering,2012.
APA	Feng, Zhihang,Li, Xinghui,Lu, Chenchen,Shen, Zhenguo,Xu, Fuliu,&Chen, Yahua.(2012).Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L..journal of bioscience and bioengineering.
MLA	Feng, Zhihang,et al."Characterization of Pseudomonas mendocina LR capable of removing nitrogen from various nitrogen-contaminated water samples when cultivated with Cyperus alternifolius L.".journal of bioscience and bioengineering (2012).