Inactivation Kinetics of Polyphenol Oxidase from Pupae of Blowfly   (Sarcophaga bullata) in the Dimethyl Sulfoxide Solution

doi:10.1007/s12010-009-8714-9

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	Inactivation Kinetics of Polyphenol Oxidase from Pupae of Blowfly (Sarcophaga bullata) in the Dimethyl Sulfoxide Solution
	Chen, Chao-Qi ; Li, Zhi-Cong ; Pan, Zhi-Zhen ; Zhu, Yu-Jing ; Yan, Ruo-Rong ; Wang, Qin ; Yan, Jiang-Hua ; Chen, Qing-Xi
刊名	应用生物化学与生物技术
	2010
关键词	Polyphenol oxidase Blowfly pupae Inactivation Kinetics Dimethyl sulfoxide MUSHROOM TYROSINASE ENZYME-ACTIVITY INHIBITION ACIDS PHENOLOXIDASE LARVAE L.
DOI	10.1007/s12010-009-8714-9
英文摘要	The effects of dimethyl sulfoxide (DMSO) on the activity of polyphenol oxidase (PPO, EC 1.14.18.1) from blowfly pupae for the oxidation of L-3,4-dihydroxyphenylalanine were studied. The results showed that low concentrations of DMSO could lead to reversible inactivation to the enzyme. The IC(50) value, the inactivator concentration leading to 50% activity lost, was estimated to be 2.35 M. Inactivation of the enzyme by DMSO was classified as mixed type. The kinetics of inactivation of PPO from blowfly pupae in the low concentrations of DMSO solution was studied using the kinetic method of the substrate reaction. The rate constants of inactivation were determined. The results show that k(+0) was much larger than k(+0)', indicating that the free enzyme molecule was more fragile than the enzyme-substrate complex in the DMSO solution. It was suggested that the presence of the substrate offers marked protection of this enzyme against inactivation by DMSO.; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; SCI(E); EI; PubMed; 0; ARTICLE; 7; 2166-2174; 160
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160716]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Chen, Chao-Qi,Li, Zhi-Cong,Pan, Zhi-Zhen,et al. Inactivation Kinetics of Polyphenol Oxidase from Pupae of Blowfly (Sarcophaga bullata) in the Dimethyl Sulfoxide Solution[J]. 应用生物化学与生物技术,2010.
APA	Chen, Chao-Qi.,Li, Zhi-Cong.,Pan, Zhi-Zhen.,Zhu, Yu-Jing.,Yan, Ruo-Rong.,...&Chen, Qing-Xi.(2010).Inactivation Kinetics of Polyphenol Oxidase from Pupae of Blowfly (Sarcophaga bullata) in the Dimethyl Sulfoxide Solution.应用生物化学与生物技术.
MLA	Chen, Chao-Qi,et al."Inactivation Kinetics of Polyphenol Oxidase from Pupae of Blowfly (Sarcophaga bullata) in the Dimethyl Sulfoxide Solution".应用生物化学与生物技术 (2010).