Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals

doi:10.1021/es4052796

CORC > 北京大学 > 城市与环境学院

	Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals
	Zhao, Yanbin ; Wang, Chen ; Xia, Shuang ; Jiang, Jieqiong ; Hu, Rui ; Yuan, Guanxiang ; Hu, Jianying
刊名	environmental science technology
	2014
关键词	ROACH RUTILUS-RUTILUS ORYZIAS-LATIPES WASTE-WATER ENVIRONMENTAL ESTROGENS SYNTHETIC ESTROGEN TRANSGENIC MEDAKA SEXUAL DISRUPTION FISH EXPOSURE ENDOCRINE
DOI	10.1021/es4052796
英文摘要	Estrogenic chemicals can induce intersex in fish species leading to disturbance of spermatogenesis and impairment of reproductive success. To overcome the shortcomings of conventional histopathological observation on intersex (low sensitivity, relatively poor accuracy, long experimental periods, as well as laborious and time-consuming), we generated a pMOSP1-EGFP transgenic medaka fish model. In this transgenic fish, the green fluorescence protein (GFP) reporter gene was derived by the regulatory elements of the OSP1 gene, which is a specific and sensitive molecular biomarker for indicating intersex occurrence in male medaka fish exposed to estrogenic chemicals. The transgenic GFP was faithfully expressed in ovaries and in testes with intersex, perfectly mimicking the expression pattern of endogenous OSP1. In intersex testis, the diameters of primary oocytes which could be distinguished by GFP fluorescence observation were as small as 10 mu m, lower than that (more than 20 mu m) which is observable by histopathology. Using the novel transgenic medaka fish, intersex was observed after 90-day exposure to 0.75 ng/L 17 alpha-ethinyloestradiol (EE2) (0-90 dph), but only at concentrations of at least 1.38 ng/L EE2 by histopathology. An effectiveness of a short-term in vivo assay for screening estrogenic chemicals that can monitor intersex appearance at early sex developmental stage (about 30 dph) in male medaka fish was also demonstrated by assessing the intersex induction of EE2, 17 beta-estradiol and 4-nonylphenol. This newly developed assay provides an enhanced ability for screening and testing estrogenic chemicals with the potential to induce intersex and studying their biological impacts.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000331774100039&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Environmental; Environmental Sciences; SCI(E); EI; PubMed; 9; ARTICLE; hujy@urban.pku.edu.cn; 4; 2413-2420; 48
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160590]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Zhao, Yanbin,Wang, Chen,Xia, Shuang,et al. Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals[J]. environmental science technology,2014.
APA	Zhao, Yanbin.,Wang, Chen.,Xia, Shuang.,Jiang, Jieqiong.,Hu, Rui.,...&Hu, Jianying.(2014).Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals.environmental science technology.
MLA	Zhao, Yanbin,et al."Biosensor Medaka for Monitoring Intersex Caused by Estrogenic Chemicals".environmental science technology (2014).