High-density lipoprotein of patients with Type 2 Diabetes Mellitus   upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in   endothelial cells: relationship with HDL-associated   sphingosine-1-phosphate

doi:10.1186/1475-2840-12-27

CORC > 北京大学 > 城市与环境学院

	High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate
	Tong, Xunliang ; Peng, Hui ; Liu, Donghui ; Ji, Liang ; Niu, Chenguang ; Ren, Jun ; Pan, Bing ; Hu, Jianying ; Zheng, Lemin ; Huang, Yining
刊名	cardiovascular diabetology
	2013
关键词	Type 2 diabetes mellitus High-density lipoprotein Sphingosine-1-phosphate Cyclooxygenase-2 Prostacyclin I-2 Endothelial cells SPHINGOSINE 1-PHOSPHATE CHOLESTEROL EFFLUX MIGRATION ATHEROSCLEROSIS DYSLIPIDEMIA PROTECTION RECEPTORS GLYCATION DISEASE RISK
DOI	10.1186/1475-2840-12-27
英文摘要	Background: Dysfunctional high-density lipoprotein (HDL) may have pro-inflammatory effects on the endothelial cells, which causes atherosclerosis in type 2 diabetes mellitus (T2DM). HDL is a major carrier of sphingosine-1-phosphate (S1P) in plasma while S1P exhibits multiple biological activities. However, potential role of HDL and S1P in T2DM remains unexplored. We hypothesized that diabetic HDL with higher contents of S1P exerts beneficial effects on the vascular system. Methods: Subjects with T2DM with or without proved large arteries atherosclerosis and normal controls (n= 15 for each group) were recruited in the present study. HDL was isolated from the subjects by ultracentrifugation. The levels of HDL-associated S1P were determined by UPLC-MS/MS. The protective function of diabetic HDL and S1P was evaluated by measuring cyclooxygenase-2 (COX-2) expression and prostacyclin I-2 (PGI-2) release by human umbilical vein endothelial cells (HUVECs) using western blot and enzyme-linked immunosorbent assay (ELISA), respectively. Results: The S1P levels in isolated HDL were significantly increased in T2DM subjects compared with controls (235.6 +/- 13.4 vs 195.0 +/- 6.4 ng/mg, P< 0.05). The diabetic HDL exerted greater protective effects on inducing COX-2 expression and PGI-2 release by HUVECs than those of control HDL (p < 0.05, p < 0.01, respectively). Pertussis toxin, a common inhibitor of G-couple protein receptors, and VPC 23019, an antagonist of S1P receptor 1 and 3 significantly attenuated HDL-induced COX-2 expression and PGI-2 release. Conclusions: Diabetic HDL carries higher level of S1P compared with normal HDL, which has the potential to contribute to protective effects on endothelial cells by inducing COX-2 expression and PGI-2 release. These findings provide a new insight of S1P function in T2DM patients, possibly leading to a new therapeutic target.; Cardiac & Cardiovascular Systems; Endocrinology & Metabolism; SCI(E); PubMed; 4; 27; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/160079]
专题	城市与环境学院
推荐引用方式 GB/T 7714	Tong, Xunliang,Peng, Hui,Liu, Donghui,et al. High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate[J]. cardiovascular diabetology,2013.
APA	Tong, Xunliang.,Peng, Hui.,Liu, Donghui.,Ji, Liang.,Niu, Chenguang.,...&Huang, Yining.(2013).High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate.cardiovascular diabetology.
MLA	Tong, Xunliang,et al."High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate".cardiovascular diabetology (2013).