Mammalian non-CG methylations are conserved and cell-type specific and   may have been involved in the evolution of transposon elements

doi:10.1038/srep32207

CORC > 北京大学 > 生命科学学院

	Mammalian non-CG methylations are conserved and cell-type specific and may have been involved in the evolution of transposon elements
	Guo, Weilong ; Zhang, Michael Q. ; Wu, Hong
刊名	SCIENTIFIC REPORTS
	2016
关键词	PRIMORDIAL GERM-CELLS NON-CPG METHYLATION PLURIPOTENT STEM-CELLS DNA METHYLATION GENOME EPIGENOME DYNAMICS RETROTRANSPOSONS SEQUENCE NAIVE
DOI	10.1038/srep32207
英文摘要	Although non-CG methylations are abundant in several mammalian cell types, their biological significance is sparsely characterized. We gathered 51 human and mouse DNA methylomes from brain neurons, embryonic stem cells and induced pluripotent stem cells, primordial germ cells and oocytes. We utilized an unbiased sub-motif prediction method and reported CW as the representative non-CG methylation context, which is distinct from CC methylation in terms of sequence context and genomic distribution. A two-dimensional comparison of non-CG methylations across cell types and species was performed. Unambiguous studies of sequence preferences and genomic region enrichment showed that CW methylation is cell-type specific and is also conserved between humans and mice. In brain neurons, it was found that active long interspersed nuclear element-1 (LINE-1) lacked CW methylations but not CG methylations. Coincidentally, both human Alu and mouse B1 elements preferred high CW methylations at specific loci during their respective evolutionary development. Last, the strand-specific distributions of CW methylations in introns and long interspersed nuclear elements are also cell-type specific and conserved. In summary, our results illustrate that CW methylations are highly conserved among species, are dynamically regulated in each cell type, and are potentially involved in the evolution of transposon elements.; China Postdoctoral Science Foundation [2015M570010]; Peking-Tsinghua Center for Life Sciences; National Basic Research Program of China [2012CB316503]; National Natural Science Foundation of China [91519326]; SCI(E); PubMed; ARTICLE; guoweilong@pku.edu.cn; hongwu@pku.edu.cn; 32207; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/493124]
专题	生命科学学院
推荐引用方式 GB/T 7714	Guo, Weilong,Zhang, Michael Q.,Wu, Hong. Mammalian non-CG methylations are conserved and cell-type specific and may have been involved in the evolution of transposon elements[J]. SCIENTIFIC REPORTS,2016.
APA	Guo, Weilong,Zhang, Michael Q.,&Wu, Hong.(2016).Mammalian non-CG methylations are conserved and cell-type specific and may have been involved in the evolution of transposon elements.SCIENTIFIC REPORTS.
MLA	Guo, Weilong,et al."Mammalian non-CG methylations are conserved and cell-type specific and may have been involved in the evolution of transposon elements".SCIENTIFIC REPORTS (2016).