Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to   Promote Restart of Stalled Replication Forks

doi:10.1074/jbc.C116.747758

CORC > 北京大学 > 生命科学学院

	Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks
	Feng, Sumin ; Zhao, Yichao ; Xu, Yixi ; Ning, Shaokai ; Huo, Wei ; Hou, Mei ; Gao, Ge ; Ji, Jianguo ; Guo, Rong ; Xu, Dongyi
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2016
关键词	DNA damage DNA damage response DNA recombination DNA repair DNA replication ATR ETAA1 Ewing tumor-associated antigen 1 RPA stalled replication fork restart ATR replication stress DNA-DAMAGE RESPONSE SINGLE-STRANDED-DNA RPA-SSDNA CHECKPOINT ACTIVATION PATHWAYS SMARCAL1 REPAIR XRCC4
DOI	10.1074/jbc.C116.747758
英文摘要	The replication protein A (RPA) complex binds single-stranded DNA generated at stalled replication forks and recruits other DNA repair proteins to promote recovery of these forks. Here, we identify Ewing tumor-associated antigen 1 (ETAA1), which has been linked to susceptibility to pancreatic cancer, as a new repair protein that is recruited to stalled forks by RPA. We demonstrate that ETAA1 interacts with RPA through two regions, each of which resembles two previously identified RPA-binding domains, RPA70N-binding motif and RPA32C-binding motif, respectively. In response to replication stress, ETAA1 is recruited to stalled forks where it colocalizes with RPA, and this recruitment is diminished when RPA is depleted. Notably, inactivation of the ETAA1 gene increases the collapse level of the stalled replication forks and decreases the recovery efficiency of these forks. Moreover, epistasis analysis shows that ETAA1 stabilizes stalled replication forks in an ataxia telangiectasia and Rad3-related protein (ATR)-independent manner. Thus, our results reveal that ETAA1 is a novel RPA-interacting protein that promotes restart of stalled replication forks.; National Basic Research Program of China [2013CB911002]; National Natural Science Foundation of China [31271435, 31370836]; SCI(E); PubMed; ARTICLE; guorong@pku.edu.cn; xudongyi@pku.edu.cn; 42; 21956-21962; 291
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492983]
专题	生命科学学院
推荐引用方式 GB/T 7714	Feng, Sumin,Zhao, Yichao,Xu, Yixi,et al. Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2016.
APA	Feng, Sumin.,Zhao, Yichao.,Xu, Yixi.,Ning, Shaokai.,Huo, Wei.,...&Xu, Dongyi.(2016).Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks.JOURNAL OF BIOLOGICAL CHEMISTRY.
MLA	Feng, Sumin,et al."Ewing Tumor-associated Antigen 1 Interacts with Replication Protein A to Promote Restart of Stalled Replication Forks".JOURNAL OF BIOLOGICAL CHEMISTRY (2016).