RNA sequencing reveals differential thermal regulation mechanisms   between sexes of Glanville fritillary butterfly in the Tianshan   Mountains, China

doi:10.1007/s11033-016-4076-x

CORC > 北京大学 > 生命科学学院

	RNA sequencing reveals differential thermal regulation mechanisms between sexes of Glanville fritillary butterfly in the Tianshan Mountains, China
	Lei, Ying ; Wang, Yang ; Ahola, Virpi ; Luo, Shiqi ; Xu, Chongren ; Wang, Rongjiang
刊名	MOLECULAR BIOLOGY REPORTS
	2016
关键词	The Glanville fritillary butterfly RNA-seq De novo Thermal tolerance HEAT-SHOCK PROTEINS ADULT DROSOPHILA-MELANOGASTER TRIBOLIUM-CASTANEUM INSECT PIGMENTATION ORCHESELLA-CINCTA GENETIC-VARIATION HSP70 EXPRESSION HIGH-TEMPERATURE MELITAEA-CINXIA STRESS-RESPONSE
DOI	10.1007/s11033-016-4076-x
英文摘要	The Glanville fritillary butterfly (Melitaea cinxia; Nymphalidae) has been extensively studied as a model species in metapopulation ecology. We investigated in the earlier studies that female butterflies exhibit higher thermal tolerance than males in the Tianshan Mountains of China. We aim to understand the molecular mechanism of differences of thermal responses between sexes. We used RNA-seq approach and performed de novo assembly of transcriptome to compare the gene expression patterns between two sexes after heat stress. All the reads were assembled into 84,376 transcripts and 72,701 unigenes. The number of differential expressed genes (DEGs) between control and heat shock samples was 175 and 268 for males and females, respectively. Heat shock proteins genes (hsps) were up-regulated in response to heat stress in both males and females. Most of the up-regulated hsps showed higher fold changes in males than in females. Females expressed more ribosomal subunit protein genes, transcriptional elongation factor genes, and methionine-rich storage protein genes, participating in protein synthesis. It indicated that protein synthesis is needed for females to replace the damaged proteins due to heat shock. In addition, aspartate decarboxylase might contribute to thermal tolerance in females. These differences in gene expression may at least partly explain the response to high temperature stress, and the fact that females exhibit higher thermal tolerance.; National Natural Science Foundation of China [30470286, 30670332, 30211130505]; Beijing Natural Science Foundation [6082012]; SCI(E); PubMed; ARTICLE; rjwang@pku.edu.cn; 12; 1423-1433; 43
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/492732]
专题	生命科学学院
推荐引用方式 GB/T 7714	Lei, Ying,Wang, Yang,Ahola, Virpi,et al. RNA sequencing reveals differential thermal regulation mechanisms between sexes of Glanville fritillary butterfly in the Tianshan Mountains, China[J]. MOLECULAR BIOLOGY REPORTS,2016.
APA	Lei, Ying,Wang, Yang,Ahola, Virpi,Luo, Shiqi,Xu, Chongren,&Wang, Rongjiang.(2016).RNA sequencing reveals differential thermal regulation mechanisms between sexes of Glanville fritillary butterfly in the Tianshan Mountains, China.MOLECULAR BIOLOGY REPORTS.
MLA	Lei, Ying,et al."RNA sequencing reveals differential thermal regulation mechanisms between sexes of Glanville fritillary butterfly in the Tianshan Mountains, China".MOLECULAR BIOLOGY REPORTS (2016).