Length-dependent flagellar growth of Vibrio alginolyticus revealed by   realtime fluorescent imaging

doi:10.7554/eLife.22140

CORC > 北京大学 > 生命科学学院

	Length-dependent flagellar growth of Vibrio alginolyticus revealed by realtime fluorescent imaging
	Chen, Meiting ; Zhao, Ziyi ; Yang, Jin ; Peng, Kai ; Baker, Matthew A. B. ; Bai, Fan ; Lo, Chien-Jung
刊名	ELIFE
	2017
关键词	PROTEIN EXPORT APPARATUS BACTERIAL FLAGELLA PROTONMOTIVE FORCE ESCHERICHIA-COLI III SECRETION ROTARY MOTOR TORQUE RING FILAMENTS TRANSLOCATION MACHINE
DOI	10.7554/eLife.22140
英文摘要	Bacterial flagella are extracellular filaments that drive swimming in bacteria. During motor assembly, flagellins are transported unfolded through the central channel in the flagellum to the growing tip. Here, we applied in vivo fluorescent imaging to monitor in real time the Vibrio alginolyticus polar flagella growth. The flagellar growth rate is found to be highly length-dependent. Initially, the flagellum grows at a constant rate (50 nm/min) when shorter than 1500 nm. The growth rate decays sharply when the flagellum grows longer, which decreases to similar to 9 nm/min at 7500 nm. We modeled flagellin transport inside the channel as a one-dimensional diffusive process with an injection force at its base. When the flagellum is short, its growth rate is determined by the loading speed at the base. Only when the flagellum grows longer does diffusion of flagellin become the rate-limiting step, dramatically reducing the growth rate. Our results shed new light on the dynamic building process of this complex extracellular structure.; National Natural Science Foundation of China [31370847, 31327901]; Human Frontier Science Program [RGP0041/2015]; Ministry of Science and Technology, Taiwan [MOST-103-2112-M-008-013-MY3]; SCI(E); ARTICLE; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/475653]
专题	生命科学学院
推荐引用方式 GB/T 7714	Chen, Meiting,Zhao, Ziyi,Yang, Jin,et al. Length-dependent flagellar growth of Vibrio alginolyticus revealed by realtime fluorescent imaging[J]. ELIFE,2017.
APA	Chen, Meiting.,Zhao, Ziyi.,Yang, Jin.,Peng, Kai.,Baker, Matthew A. B..,...&Lo, Chien-Jung.(2017).Length-dependent flagellar growth of Vibrio alginolyticus revealed by realtime fluorescent imaging.ELIFE.
MLA	Chen, Meiting,et al."Length-dependent flagellar growth of Vibrio alginolyticus revealed by realtime fluorescent imaging".ELIFE (2017).