Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging

doi:10.1038/cr.2017.9

CORC > 北京大学 > 生命科学学院

	Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging
	Zhou, Yuexin ; Wang, Ping ; Tian, Feng ; Gao, Ge ; Huang, Lei ; Wei, Wensheng ; Xie, X. Sunney
刊名	CELL RESEARCH
	2017
关键词	Painting chromosome imaging GENOMIC LOCI SYSTEM
DOI	10.1038/cr.2017.9
英文摘要	Visualization of chromosome shapes and dynamics in a live cell is highly desirable and necessary in many areas of cell biology. For example, the copy number of a particular chromosome in cancer cells is often abnormal (e.g., more than two), and therefore probing chromosome copy numbers can aid cancer diagnosis. During interphase, each chromosome exists in its own territory in the nucleus, which can be imaged by fluorescence in situ hybridization (FISH) using sequence-specific probes of different colors [1,2]. However, such chromosome painting has only been possible in fixed cells, and is not suitable for dynamic monitoring of live cells. Therefore, it would be valuable to visualize DNA replication of one chromosome during interphase, and follow chromosome dynamics in the M phase.; National Natural Science Foundation of China [NSFC 21390412, NSFC21327808, NSFC31430025, NSFC31170126, NSFC81471909]; National Basic Research Program of China [2015AA020407]; SCI(E); 中国科学引文数据库(CSCD); LETTER; 2; 298-301; 27
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/475409]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhou, Yuexin,Wang, Ping,Tian, Feng,et al. Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging[J]. CELL RESEARCH,2017.
APA	Zhou, Yuexin.,Wang, Ping.,Tian, Feng.,Gao, Ge.,Huang, Lei.,...&Xie, X. Sunney.(2017).Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging.CELL RESEARCH.
MLA	Zhou, Yuexin,et al."Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging".CELL RESEARCH (2017).