Reduction of translating ribosomes enables Escherichia coli to maintain   elongation rates during slow growth

doi:10.1038/nmicrobiol.2016.231

CORC > 北京大学 > 生命科学学院

	Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth
	Dai, Xiongfeng ; Zhu, Manlu ; Warren, Mya ; Balakrishnan, Rohan ; Patsalo, Vadim ; Okano, Hiroyuki ; Williamson, James R. ; Fredrick, Kurt ; Wang, Yi-Ping ; Hwa, Terence
刊名	NATURE MICROBIOLOGY
	2017
关键词	PROTEIN-SYNTHESIS BETA-GALACTOSIDASE GENE-EXPRESSION RIBONUCLEIC-ACID CELL-GROWTH MASS-SPECTROMETRY IN-VIVO B-R INHIBITION BACTERIA
DOI	10.1038/nmicrobiol.2016.231
英文摘要	Bacteria growing under different conditions experience a broad range of demand on the rate of protein synthesis, which profoundly affects cellular resource allocation. During fast growth, protein synthesis has long been known to be modulated by adjusting the ribosome content, with the vast majority of ribosomes engaged at a near-maximal rate of elongation. Here, we systematically characterize protein synthesis by Escherichia coli, focusing on slow-growth conditions. We establish that the translational elongation rate decreases as growth slows, exhibiting a Michaelis-Menten dependence on the abundance of the cellular translational apparatus. However, an appreciable elongation rate is maintained even towards zero growth, including the stationary phase. This maintenance, critical for timely protein synthesis in harsh environments, is accompanied by a drastic reduction in the fraction of active ribosomes. Interestingly, well-known antibiotics such as chloramphenicol also cause a substantial reduction in the pool of active ribosomes, instead of slowing down translational elongation as commonly thought.; NIH [R01GM109069, R01GM072528, GM118850]; National Natural Science Fund of the People's Republic of China (NSFC) [31530081]; China Scholarship Council (CSC) [201306010039]; SCI(E); ARTICLE; 2; 2
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/475373]
专题	生命科学学院
推荐引用方式 GB/T 7714	Dai, Xiongfeng,Zhu, Manlu,Warren, Mya,et al. Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth[J]. NATURE MICROBIOLOGY,2017.
APA	Dai, Xiongfeng.,Zhu, Manlu.,Warren, Mya.,Balakrishnan, Rohan.,Patsalo, Vadim.,...&Hwa, Terence.(2017).Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth.NATURE MICROBIOLOGY.
MLA	Dai, Xiongfeng,et al."Reduction of translating ribosomes enables Escherichia coli to maintain elongation rates during slow growth".NATURE MICROBIOLOGY (2017).