IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair   by Homologous Recombination in Arabidopsis

doi:10.1105/tpc.16.00769

CORC > 北京大学 > 生命科学学院

	IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis
	Liu, Mingming ; Ba, Zhaoqing ; Costa-Nunes, Pedro ; Wei, Wei ; Li, Lanxia ; Kong, Fansi ; Li, Yan ; Chai, Jijie ; Pontes, Olga ; Qi, Yijun
刊名	PLANT CELL
	2017
关键词	REPLICATION PROTEIN-A DIFFERENT PATHWAYS DAMAGE RESPONSE IONIZING-RADIATION NONCODING RNA METHYLATION PLANTS THALIANA COMPLEX ROLES
DOI	10.1105/tpc.16.00769
英文摘要	Repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genome integrity. We previously showed that DSB-induced small RNAs (diRNAs) facilitate homologous recombination-mediated DSB repair in Arabidopsis thaliana. Here, we show that INVOLVED IN DE NOVO2 (IDN2), a double-stranded RNA binding protein involved in small RNA-directed DNA methylation, is required for DSB repair in Arabidopsis. We find that IDN2 interacts with the heterotrimeric replication protein A (RPA) complex. Depletion of IDN2 or the diRNA binding ARGONAUTE2 leads to increased accumulation of RPA at DSB sites and mislocalization of the recombination factor RAD51. These findings support a model in which IDN2 interacts with RPA and facilitates the release of RPA from single-stranded DNA tails and subsequent recruitment of RAD51 at DSB sites to promote DSB repair.; National Key Research and Development Program of China [2016YFA0500800]; National Science Foundation of China [31421001, 31330042]; SCI(E); ARTICLE; 3; 589-599; 29
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474805]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Mingming,Ba, Zhaoqing,Costa-Nunes, Pedro,et al. IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis[J]. PLANT CELL,2017.
APA	Liu, Mingming.,Ba, Zhaoqing.,Costa-Nunes, Pedro.,Wei, Wei.,Li, Lanxia.,...&Qi, Yijun.(2017).IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis.PLANT CELL.
MLA	Liu, Mingming,et al."IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis".PLANT CELL (2017).