Stiffness-Controlled Thermoresponsive Hydrogels for Cell Harvesting with   Sustained Mechanical Memory

doi:10.1002/adhm.201601152

CORC > 北京大学 > 生命科学学院

	Stiffness-Controlled Thermoresponsive Hydrogels for Cell Harvesting with Sustained Mechanical Memory
	Fan, Xingliang ; Zhu, Lu ; Wang, Ke ; Wang, Bingjie ; Wu, Yaozu ; Xie, Wei ; Huang, Chengyu ; Chan, Barbara Pui ; Du, Yanan
刊名	ADVANCED HEALTHCARE MATERIALS
	2017
关键词	MESENCHYMAL STEM-CELLS HISTONE ACETYLATION PATTERNS PROTEIN EXTRACTION METHODS EXTRACELLULAR-MATRIX DNA METHYLATION COLLAGEN CONSTRUCTS ADIPOSE-TISSUE FOCAL ADHESION DIFFERENTIATION CULTURE
DOI	10.1002/adhm.201601152
英文摘要	Most mechanobiological investigations focused on in situ mechanical regulation of cells on stiffness-controlled substrates with few downstream applications, as it is still challenging to harvest and expand mechanically primed cells by enzymatic digestion (e.g., trypsin) without interrupting cellular mechanical memory between passages. This study develops thermoresponsive hydrogels with controllable stiffness to generate mechanically primed cells with intact mechanical memory for augmented wound healing. No significant cellular property alteration of the fibroblasts primed on thermo-responsive hydrogels with varied stiffness has been observed through thermoresponsive harvesting. When reseeding the harvested cells for further evaluation, softer hydrogels are proven to better sustain the mechanical priming effects compared to rigid tissue culture plate, which indicates that both the stiffness-controlled substrate and thermoresponsive harvesting are required to sustain cellular mechanical memory between passages. Moreover, epigenetics analysis reveals that thermoresponsive harvesting could reduce the rearrangement and loss of chromatin proteins compared to that of trypsinization. In vivo wound healing using mechanically primed fibroblasts shows featured epithelium and sebaceous glands, which indicates augmented skin recovery compared with trypsinized fibroblasts. Thus, the thermoresponsive hydrogel-based cell harvesting system offers a powerful tool to investigate mechanobiology between cell passages and produces abundant cells with tailored mechanical priming properties for cell-based applications.; National Natural Science Foundation of China [51273106, 11372243]; Beijing Natural Science Foundation [7162210]; NSFC/RGC Joint Research Scheme [N_HKU713/14, 51461165302]; Chinese Postdoctoral Research Funding [2015M570116]; SCI(E); ARTICLE; 5; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474639]
专题	生命科学学院
推荐引用方式 GB/T 7714	Fan, Xingliang,Zhu, Lu,Wang, Ke,et al. Stiffness-Controlled Thermoresponsive Hydrogels for Cell Harvesting with Sustained Mechanical Memory[J]. ADVANCED HEALTHCARE MATERIALS,2017.
APA	Fan, Xingliang.,Zhu, Lu.,Wang, Ke.,Wang, Bingjie.,Wu, Yaozu.,...&Du, Yanan.(2017).Stiffness-Controlled Thermoresponsive Hydrogels for Cell Harvesting with Sustained Mechanical Memory.ADVANCED HEALTHCARE MATERIALS.
MLA	Fan, Xingliang,et al."Stiffness-Controlled Thermoresponsive Hydrogels for Cell Harvesting with Sustained Mechanical Memory".ADVANCED HEALTHCARE MATERIALS (2017).