Modular electron-transport chains from eukaryotic organelles function to   support nitrogenase activity

doi:10.1073/pnas.1620058114

CORC > 北京大学 > 生命科学学院

	Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity
	Yang, Jianguo ; Xie, Xiaqing ; Yang, Mingxuan ; Dixon, Ray ; Wang, Yi-Ping
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2017
关键词	nitrogen fixation electron transport plant organelles nitrogenase engineering PLANT-TYPE FERREDOXINS IRON-ONLY NITROGENASE FE-PROTEIN RHODOBACTER-CAPSULATUS AZOTOBACTER-VINELANDII GENE-PRODUCT FIXATION REDUCTASE OXIDOREDUCTASE CYANOBACTERIA
DOI	10.1073/pnas.1620058114
英文摘要	A large number of genes are necessary for the biosynthesis and activity of the enzyme nitrogenase to carry out the process of biological nitrogen fixation (BNF), which requires large amounts of ATP and reducing power. The multiplicity of the genes involved, the oxygen sensitivity of nitrogenase, plus the demand for energy and reducing power, are thought to be major obstacles to engineering BNF into cereal crops. Genes required for nitrogen fixation can be considered as three functional modules encoding electron-transport components (ETCs), proteins required for metal cluster biosynthesis, and the "core" nitrogenase apoenzyme, respectively. Among these modules, the ETC is important for the supply of reducing power. In this work, we have used Escherichia coli as a chassis to study the compatibility between molybdenum and the iron-only nitrogenases with ETC modules from target plant organelles, including chloroplasts, root plastids, and mitochondria. We have replaced an ETC module present in diazotrophic bacteria with genes encoding ferredoxin-NADPH oxidoreductases (FNRs) and their cognate ferredoxin counterparts from plant organelles. We observe that the FNR-ferredoxin module from chloroplasts and root plastids can support the activities of both types of nitrogenase. In contrast, an analogous ETC module from mitochondria could not function in electron transfer to nitrogenase. However, this incompatibility could be overcome with hybrid modules comprising mitochondrial NADPH-dependent adrenodoxin oxidoreductase and the Anabaena ferredoxins FdxH or FdxB. We pinpoint endogenous ETCs from plant organelles as power supplies to support nitrogenase for future engineering of diazotrophy in cereal crops.; National Science Foundation of China (NSFC) [31530081]; 973 National Key Basic Research Program in China [2015CB755700]; China Postdoctoral Science Foundation [2015M580014]; State Key Laboratory of Protein and Plant Gene Research Grant [B02]; NSFC National Science Fund for Distinguished Young Scholars [39925017]; U.K. Biotechnology and Biological Sciences Research Council [BB/J004553/1]; Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences; SCI(E); ARTICLE; 12; E2460-E2465; 114
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474528]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, Jianguo,Xie, Xiaqing,Yang, Mingxuan,et al. Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017.
APA	Yang, Jianguo,Xie, Xiaqing,Yang, Mingxuan,Dixon, Ray,&Wang, Yi-Ping.(2017).Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
MLA	Yang, Jianguo,et al."Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017).