Noncanonical role of Arabidopsis COP1/SPA complex in repressing   BIN2-mediated PIF3 phosphorylation and degradation in darkness

doi:10.1073/pnas.1700850114

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	Noncanonical role of Arabidopsis COP1/SPA complex in repressing BIN2-mediated PIF3 phosphorylation and degradation in darkness
	Ling, Jun-Jie ; Li, Jian ; Zhu, Danmeng ; Deng, Xing Wang
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2017
关键词	photomorphogenesis COP1/SPA complex posttranscriptional regulation PHYTOCHROME-INTERACTING FACTORS PROTEASOME REGULATORY COMPLEX E3 LIGASE TRANSCRIPTION FACTORS MEDIATED DEGRADATION GENE-EXPRESSION LIGHT CONTROL COP9 COMPLEX PHOTOMORPHOGENESIS SEEDLINGS
DOI	10.1073/pnas.1700850114
英文摘要	The E3 ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) has been known to mediate key signaling factors for degradation via the ubiquitin/26S proteasome pathway in both plants and animals. Here, we report a noncanonical function of Arabidopsis COP1, the central repressor of photomorphogenesis, in the form of a COP1/SUPPRESSOR of phyA-105 (SPA) complex. We show that the COP1/SPA complex associates with and stabilizes PHYTOCHROME INTERACTING FACTOR 3 (PIF3) to repress photomorphogenesis in the dark. We identify the GSK3-like kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2) as a kinase of PIF3, which induces PIF3 degradation via 26S proteasome during skotomorphogenesis. Mutations on two typical BIN2 phosphorylation motifs of PIF3 lead to a strong stabilization of the protein in the dark. We further show that the COP1/SPA complex promotes PIF3 stability by repressing BIN2 activity. Intriguingly, without affecting BIN2 expression, the COP1/SPA complex modulates BIN2 activity through interfering with BIN2-PIF3 interaction, thereby inhibiting BIN2-mediated PIF3 phosphorylation and degradation. Taken together, our results suggest another paradigm for COP1/SPA complex action in the precise control of skotomorphogenesis.; National Natural Science Foundation of China [31330048, 31621001, 91540105]; National Basic Research Program (973 Program) Grant [2012CB910900]; NIH [GM47850]; State Key Laboratory of Protein and Plant Gene Research at Peking University; Peking-Tsinghua Center for Life Sciences; SCI(E); ARTICLE; 13; 3539-3544; 114
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/474462]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ling, Jun-Jie,Li, Jian,Zhu, Danmeng,et al. Noncanonical role of Arabidopsis COP1/SPA complex in repressing BIN2-mediated PIF3 phosphorylation and degradation in darkness[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017.
APA	Ling, Jun-Jie,Li, Jian,Zhu, Danmeng,&Deng, Xing Wang.(2017).Noncanonical role of Arabidopsis COP1/SPA complex in repressing BIN2-mediated PIF3 phosphorylation and degradation in darkness.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
MLA	Ling, Jun-Jie,et al."Noncanonical role of Arabidopsis COP1/SPA complex in repressing BIN2-mediated PIF3 phosphorylation and degradation in darkness".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017).