Hierarchical assembly of centriole subdistal appendages via centrosome   binding proteins CCDC120 and CCDC68

doi:10.1038/ncomms15057

CORC > 北京大学 > 生命科学学院

	Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68
	Huang, Ning ; Xia, Yuqing ; Zhang, Donghui ; Wang, Song ; Bao, Yitian ; He, Runsheng ; Teng, Junlin ; Chen, Jianguo
刊名	NATURE COMMUNICATIONS
	2017
关键词	MOTHER CENTRIOLE GAMMA-TUBULIN MICROTUBULE NUCLEATION CILIA BIOGENESIS BASAL BODIES NINEIN CILIOGENESIS INTERACTS COMPONENT EB1
DOI	10.1038/ncomms15057
英文摘要	In animal cells, the centrosome is the main microtubule-organizing centre where microtubules are nucleated and anchored. The centriole subdistal appendages (SDAs) are the key structures that anchor microtubules in interphase cells, but the composition and assembly mechanisms of SDAs are not well understood. Here, we reveal that centrosomebinding proteins, coiled-coil domain containing (CCDC) 120 and CCDC68 are two novel SDA components required for hierarchical SDA assembly in human cells. CCDC120 is anchored to SDAs by ODF2 and recruits CEP170 and Ninein to the centrosome through different coiled-coil domains at its N terminus. CCDC68 is a CEP170-interacting protein that competes with CCDC120 in recruiting CEP170 to SDAs. Furthermore, CCDC120 and CCDC68 are required for centrosome microtubule anchoring. Our findings elucidate the molecular basis for centriole SDA hierarchical assembly and microtubule anchoring in human interphase cells.; National Natural Science Foundation of China [31630092, 31171283]; National Key Research and Development Program of China, Stem Cell and Translational Research [2016YFA0100501]; SCI(E); ARTICLE; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/473954]
专题	生命科学学院
推荐引用方式 GB/T 7714	Huang, Ning,Xia, Yuqing,Zhang, Donghui,et al. Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68[J]. NATURE COMMUNICATIONS,2017.
APA	Huang, Ning.,Xia, Yuqing.,Zhang, Donghui.,Wang, Song.,Bao, Yitian.,...&Chen, Jianguo.(2017).Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68.NATURE COMMUNICATIONS.
MLA	Huang, Ning,et al."Hierarchical assembly of centriole subdistal appendages via centrosome binding proteins CCDC120 and CCDC68".NATURE COMMUNICATIONS (2017).