Ligand- and voltage-gated Ca2+ channels differentially regulate the mode   of vesicular neuropeptide release in mammalian sensory neurons

doi:10.1126/scisignal.aal1683

CORC > 北京大学 > 生命科学学院

	Ligand- and voltage-gated Ca2+ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons
	Wang, Yeshi ; Wu, Qihui ; Hu, Meiqin ; Liu, Bin ; Chai, Zuying ; Huang, Rong ; Wang, Yuan ; Xu, Huadong ; Zhou, Li ; Zheng, Lianghong ; Wang, Changhe ; Zhou, Zhuan
刊名	SCIENCE SIGNALING
	2017
关键词	ROOT GANGLION NEURONS KISS-AND-RUN ADRENAL CHROMAFFIN CELLS TRANSMITTER RELEASE DEPENDENT SECRETION ION CHANNELS EXOCYTOSIS CALCIUM ACTIVATION ASTROCYTES
DOI	10.1126/scisignal.aal1683
英文摘要	Neuropeptides released from dorsal root ganglion (DRG) neurons play essential roles in the neurotransmission of sensory inputs, including those underlying nociception and pathological pain. Neuropeptides are released from intracellular vesicles through two modes: a partial release mode called "kiss-and-run" (KAR) and a full release mode called "full fusion-like" (FFL). Using total internal reflection fluorescence (TIRF) microscopy, we traced the release of pH-sensitive green fluorescent protein-tagged neuropeptide Y (pHluorin-NPY) from individual dense-core vesicles in the soma and axon of single DRG neurons after Ca2+ influx through either voltage-gated Ca2+ channels (VGCCs) or ligand-gated transient receptor potential vanilloid 1 (TRPV1) channels. We found that Ca2+ influx through VGCCs stimulated FFL and a greater single release of neuropeptides. In contrast, Ca2+ influx through TRPV1 channels stimulated KAR and a pulsed but prolonged release of neuropeptides that was partially mediated by Dynamin 1, which limits fusion pore expansion. Suppressing the Ca2+ gradient to an extent similar to that seen after TRPV1 activation abolished the VGCC preference for FFL. The findings suggest that by generating a steeper Ca2+ gradient, VGCCs promote a more robust fusion pore opening that facilitates FFL. Thus, KAR and FFL release modes are differentially regulated by the two principal types of Ca2+-permeable channels in DRG neurons.; National Basic Research Program of China [2012CB518006, 2016YFA0500401]; National Natural Science Foundation of China [31228010, 31171026, 31100597, 31327901, 31221002, 31330024, 31400708, 31521062, 31670843]; Peking-Tsinghua Center for Life Sciences; SCI(E); ARTICLE; 484; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472751]
专题	生命科学学院心理与认知科学学院
推荐引用方式 GB/T 7714	Wang, Yeshi,Wu, Qihui,Hu, Meiqin,et al. Ligand- and voltage-gated Ca2+ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons[J]. SCIENCE SIGNALING,2017.
APA	Wang, Yeshi.,Wu, Qihui.,Hu, Meiqin.,Liu, Bin.,Chai, Zuying.,...&Zhou, Zhuan.(2017).Ligand- and voltage-gated Ca2+ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons.SCIENCE SIGNALING.
MLA	Wang, Yeshi,et al."Ligand- and voltage-gated Ca2+ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons".SCIENCE SIGNALING (2017).