Multicolor Super-resolution Fluorescence Microscopy with Blue and   Carmine Small Photoblinking Polymer Dots

doi:10.1021/acsnano.7b02893

CORC > 北京大学 > 生命科学学院

	Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots
	Chen, Xuanze ; Liu, Zhihe ; Li, Rongqin ; Shan, Chunyan ; Zeng, Zhiping ; Xue, Boxin ; Yuan, Weihong ; Mo, Chi ; Xi, Peng ; Wu, Changfeng ; Sun, Yujie
刊名	ACS NANO
	2017
关键词	multicolor super-resolution imaging super-resolution optical fluctuation imaging blue and carmine small polymer dots photoblinking bioimaging FLUCTUATION IMAGING SOFI BIOLOGICAL APPLICATIONS QUANTUM DOTS ORGANIC-DYES NANOPARTICLES ACETYLATION NANOSCOPY EMISSION PROTEIN DESIGN
DOI	10.1021/acsnano.7b02893
英文摘要	Advances in the development of small photoblinking semiconducting polymer dots (Pdots) have attracted great interest for use in super-resolution microscopy. However, multicolor super-resolution imaging using conventional small photoblinking Pdots remains a challenge due to their limited color choice, broad emission spectrum, and heavy spectrum crosstalk. Here, we introduce two types of small photoblinking Pdots with different colors and relatively narrow emission spectra: blue PFO Pdots and carmine PFTBTS Pdots for blinking-based statistical nanoscopy. Both of these probes feature ultrahigh single-particle brightness, very strong photostability, superior biocompatibility, and robust fluorescence fluctuation. In addition, these small photoblinking Pdots serve as excellent labels for dual-color super resolution optical fluctuation imaging (SOFT) of specific subcellular structures, indicating their promise for long-term multicolor SOFI nanoscopy with high spatiotemporal resolution.; National Natural Science Foundation of China [61475010]; Key Program of National Natural Science Foundation of China [61335001, 21573013, 21390412, 31271423, 31327901]; Thousand Young Talents Program; "863" Program [SS2015AA020406]; SCI(E); ARTICLE; 8; 8084-8091; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471679]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Chen, Xuanze,Liu, Zhihe,Li, Rongqin,et al. Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots[J]. ACS NANO,2017.
APA	Chen, Xuanze.,Liu, Zhihe.,Li, Rongqin.,Shan, Chunyan.,Zeng, Zhiping.,...&Sun, Yujie.(2017).Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots.ACS NANO.
MLA	Chen, Xuanze,et al."Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots".ACS NANO (2017).