Engineering Escherichia coli to bind to cyanobacteria

doi:10.1016/j.jbiosc.2016.09.010

CORC > 北京大学 > 生命科学学院

	Engineering Escherichia coli to bind to cyanobacteria
	Zhang Zijian ; Meng Liuyi ; Ni Congjian ; Yao Lanqiu ; Zhang Fengyu ; Jin Yuji ; Mu Xuelang ; Zhu Shiyu ; Lu Xiaoyu ; Liu Shiyu ; Yu Congyu ; Wang Chenggong ; Zheng Pu ; Wu Jie ; Kang Li ; Zhang Haoqian M ; Ouyang Qi
刊名	Journal of bioscience and bioengineering
	2016
关键词	Binding,Cyanobacteria,Escherichia coli,Lectin,Microvirin,Surface display
DOI	10.1016/j.jbiosc.2016.09.010
英文摘要	We engineered Escherichia coli cells to bind to cyanobacteria by heterologously producing and displaying lectins of the target cyanobacteria on their surface. To prove the efficacy of our approach, we tested this design on Microcystis aeruginosa with microvirin (Mvn), the lectin endogenously produced by this cyanobacterium. The coding sequence of Mvn was C-terminally fused to the ice nucleation protein NC (INPNC) gene and expressed in E.?coli. Results showed that E.?coli cells expressing the INPNC::Mvn fusion protein were able to bind to M.?aeruginosa and the average number of E.?coli cells bound to each cyanobacterial cell was enhanced 8-fold. Finally, a computational model was developed to simulate the binding reaction and help reconstruct the binding parameters. To our best knowledge, this is the first report on the binding of two organisms in liquid culture mediated by the surface display of lectins and it may serve as a novel approach to mediate microbial adhesion.Copyright ? 2016 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.; PubMed
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457304]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang Zijian,Meng Liuyi,Ni Congjian,et al. Engineering Escherichia coli to bind to cyanobacteria[J]. Journal of bioscience and bioengineering,2016.
APA	Zhang Zijian.,Meng Liuyi.,Ni Congjian.,Yao Lanqiu.,Zhang Fengyu.,...&Ouyang Qi.(2016).Engineering Escherichia coli to bind to cyanobacteria.Journal of bioscience and bioengineering.
MLA	Zhang Zijian,et al."Engineering Escherichia coli to bind to cyanobacteria".Journal of bioscience and bioengineering (2016).