Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis

doi:10.1016/j.molp.2015.10.001

CORC > 北京大学 > 生命科学学院

	Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis
	Dongjin Qing ; Zhu Yang ; Mingzhe Li ; Wai Shing Wong ; Guangyu Guo ; Shichang Liu ; Hongwei Guo
刊名	分子植物（英文版）
	2016
关键词	ethylene signaling quantitative PTM proteomics aquaporin 15N stable isotope labeling in Arabidopsis (SILIA) mass spectrometry water transport PLASMA-MEMBRANE AQUAPORINS DEPENDENT PROTEIN-KINASE F-BOX PROTEINS MASS-SPECTROMETRY TRANSCRIPTION FACTOR SOYBEAN NODULIN-26 INTRINSIC PROTEIN HORMONE ETHYLENE PETAL EXPANSION CHANNEL PROTEIN
DOI	10.1016/j.molp.2015.10.001
英文摘要	Ethylene participates in the regulation of numerous cellular events and biological processes, including water loss, during leaf and flower petal wilting. The diverse ethylene responses may be regulated via dynamic interplays between protein phosphorylation/dephosphorylation and ubiquitin/26S proteasome-mediated protein degradation and protease cleavage. To address how ethylene alters protein phosphorylation through multi-furcated signaling pathways, we performed a ~(15)N stable isotope labelling-based, differential, and quantitative phosphoproteomics study on air- and ethylene-treated ethylene-insensitive Arabidopsis double loss-of-function mutant ein3-1/eil1-1. Among 535 non-redundant phosphopeptides identified, two and four phosphopeptides were up- and downregulated by ethylene, respectively. Ethyleneregulated phosphorylation of aquaporin PIP2;1 is positively correlated with the water flux rate and water loss in leaf. Genetic studies in combination with quantitative proteomics, immunoblot analysis, protoplast swelling/shrinking experiments, and leaf water loss assays on the transgenic plants expressing both the wild-type and S280A/S283A-mutated PIP2;1 in the both Col-0 and ein3eil1 genetic backgrounds suggest that ethylene increases water transport rate in Arabidopsis cells by enhancing S280/S283 phosphorylation at the C terminus of PIP2;1. Unknown kinase and/or phosphatase activities may participate in the initial upregulation independent of the cellular functions of EIN3/EIL1. This finding contributes to our understanding of ethylene-regulated leaf wilting that is commonly observed during post-harvest storage of plant organs.; This research work was supported by grants,the HKUST internal support from both the Energy Institute of HKUST,the Croucher Foundation CAS-HKUST Joint Laboratory matching fund,as well as the Equipment fund; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); boningli@ust.hk; 1; 158-174; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/449933]
专题	生命科学学院
推荐引用方式 GB/T 7714	Dongjin Qing,Zhu Yang,Mingzhe Li,et al. Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis[J]. 分子植物（英文版）,2016.
APA	Dongjin Qing.,Zhu Yang.,Mingzhe Li.,Wai Shing Wong.,Guangyu Guo.,...&Hongwei Guo.(2016).Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis.分子植物（英文版）.
MLA	Dongjin Qing,et al."Quantitative and Functional Phosphoproteomic Analysis Reveals that Ethylene Regulates Water Transport via the C-Terminal Phosphorylation of Aquaporin PIP2;1 in Arabidopsis".分子植物（英文版） (2016).