Pluripotent stem cells induced from mouse neural stem cells and small   intestinal epithelial cells by small molecule compounds

doi:10.1038/cr2015.142

CORC > 北京大学 > 生命科学学院

	Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds
	Ye, Junqing ; Ge, Jian ; Zhang, Xu ; Cheng, Lin ; Zhang, Zhengyuan ; He, Shan ; Wang, Yuping ; Lin, Hua ; Yang, Weifeng ; Liu, Junfang ; Zhao, Yang ; Deng, Hongkui
刊名	CELL RESEARCH
	2016
关键词	reprogramming CiPSC cell type fine-tuning HUMAN PERIPHERAL-BLOOD SOMATIC-CELLS PROGENITOR CELLS HUMAN FIBROBLASTS NERVOUS-SYSTEM SELF-RENEWAL ADULT-MOUSE EXPRESSION GENERATION STATE
DOI	10.1038/cr2015.142
英文摘要	Recently, we reported a chemical approach to generate pluripotent stem cells from mouse fibroblasts. However, whether chemically induced pluripotent stem cells (CiPSCs) can be derived from other cell types remains to be demonstrated. Here, using lineage tracing, we first verify the generation of CiPSCs from fibroblasts. Next, we demonstrate that neural stem cells (NSCs) from the ectoderm and small intestinal epithelial cells (IECs) from the endoderm can be chemically reprogrammed into pluripotent stem cells. CiPSCs derived from NSCs and IECs resemble mouse embryonic stem cells in proliferation rate, global gene expression profile, epigenetic status, self-renewal and differentiation capacity, and germline transmission competency. Interestingly, the pluripotency gene Sall4 is expressed at the initial stage in the chemical reprogramming process from different cell types, and the same core small molecules are required for the reprogramming, suggesting conservation in the molecular mechanism underlying chemical reprogramming from these diverse cell types. Our analysis also shows that the use of these small molecules should be fine-tuned to meet the requirement of reprogramming from different cell types. Together, these findings demonstrate that full chemical reprogramming approach can be applied in cells of different tissue origins and suggest that chemical reprogramming is a promising strategy with the potential to be extended to more initial types.; BeiHao Stem Cell and Regenerative Medicine Translational Research Institute; SCI(E); PubMed; 中国科技核心期刊(ISTIC); ARTICLE; yangzhao@pku.edu.cn; hongkui_deng@pku.edu.cn; 1; 34-45; 26
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435672]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ye, Junqing,Ge, Jian,Zhang, Xu,et al. Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds[J]. CELL RESEARCH,2016.
APA	Ye, Junqing.,Ge, Jian.,Zhang, Xu.,Cheng, Lin.,Zhang, Zhengyuan.,...&Deng, Hongkui.(2016).Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds.CELL RESEARCH.
MLA	Ye, Junqing,et al."Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds".CELL RESEARCH (2016).