CRL4(Wdr70) regulates H2B monoubiquitination and facilitates   Exo1-dependent resection

doi:10.1038/ncomms11364

CORC > 北京大学 > 生命科学学院

	CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection
	Zeng, Ming ; Ren, Laifeng ; Mizuno, Ken&apos ; Nestoras, Konstantinos ; Wang, Haibin ; Tang, Zizhi ; Guo, Liandi ; Kong, Daochun ; Hu, Qiwen ; He, Qun ; Du, Lilin ; Carr, Antony M. ; Liu, Cong ; Ichi
刊名	NATURE COMMUNICATIONS
	2016
关键词	DOUBLE-STRAND BREAKS CHECKPOINT PROTEIN CRB2 HOMOLOGOUS RECOMBINATION UBIQUITIN LIGASE DNA-DAMAGE SCHIZOSACCHAROMYCES-POMBE HISTONE H2B FISSION YEAST RIBONUCLEOTIDE REDUCTASE END RESECTION
DOI	10.1038/ncomms11364
英文摘要	Double-strand breaks repaired by homologous recombination (HR) are first resected to form single-stranded DNA, which binds replication protein A (RPA). RPA attracts mediators that load the Rad51 filament to promote strand invasion, the defining feature of HR. How the resection machinery navigates nucleosome-packaged DNA is poorly understood. Here we report that in Schizosaccharomyces bombe a conserved DDB1-CUL4-associated factor (DCAF), Wdr70, is recruited to DSBs as part of the Cullin4-DDB1 ubiquitin ligase (CRL4(Wdr70)) and stimulates distal H2B lysine 119 mono-ubiquitination (uH2B). Wdr70 deletion, or uH2B loss, results in increased loading of the checkpoint adaptor and resection inhibitor Crb2(53BP1), decreased Exo1 association and delayed resection. Wdr70 is dispensable for resection upon Crb253BP1 loss, or when the Set9 methyltransferase that creates docking sites for Crb2 is deleted. Finally, we establish that this histone regulatory cascade similarly controls DSB resection in human cells.; Ministry of Science and Technology of China [2011CB966200, 2013CB911000]; NSF China [31171319, 31471276]; Department of Science and Technology of Sichuan Province [2011SZ0002, 2012JQ0005]; Medical Research Council [G1100074]; Worldwide Cancer Research [12-1118]; [PCSIRT0935]; SCI(E); PubMed; ARTICLE; a.m.carr@sussex.ac.uk; congliu@scu.edu.cn; 11364; 7
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434034]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zeng, Ming,Ren, Laifeng,Mizuno, Ken&apos,et al. CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection[J]. NATURE COMMUNICATIONS,2016.
APA	Zeng, Ming.,Ren, Laifeng.,Mizuno, Ken&apos.,Nestoras, Konstantinos.,Wang, Haibin.,...&Ichi.(2016).CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection.NATURE COMMUNICATIONS.
MLA	Zeng, Ming,et al."CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection".NATURE COMMUNICATIONS (2016).