Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation

doi:10.1039/c6nr00324a

CORC > 北京大学 > 生命科学学院

	Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation
	Chen, Xuanze ; Zong, Weijian ; Li, Rongqin ; Zeng, Zhiping ; Zhao, Jia ; Xi, Peng ; Chen, Liangyi ; Sun, Yujie
刊名	NANOSCALE
	2016
关键词	IMAGING SOFI QUANTUM DOTS SUPERRESOLUTION MICROSCOPY PROTEIN
DOI	10.1039/c6nr00324a
英文摘要	Advances in light-sheet microscopy have enabled the fast three-dimensional (3D) imaging of live cells and bulk specimens with low photodamage and phototoxicity. Combining light-sheet illumination with super-resolution imaging is expected to resolve subcellular structures. Actually, such kind of super-resolution light-sheet microscopy was recently demonstrated using a single-molecule localization algorithm. However, the imaging depth and temporal resolution of this method are limited owing to the requirements of precise single molecule localization and reconstruction. In this work, we present two-photon super-resolution light-sheet imaging via stochastic optical fluctuation imaging (2PLS-SOFI), which acquires high spatiotemporal resolution and excellent optical sectioning ability. 2PLS-SOFI is based on non-linear excitation of fluctuation/blinking probes using our recently developed fast two-photon three-axis digital scanned light-sheet microscope (2P3A-DSLM), which enables both deep penetration and thin sheet of light. Overall, 2PLS-SOFI demonstrates up to 3-fold spatial resolution enhancement compared with conventional two-photon light-sheet (2PLS) microscopy and about 40-fold temporal resolution enhancement compared with individual molecule localization-selective plane illumination microscopy (IML-SPIM). Therefore, 2PLS-SOFI is promising for 3D long-term, deep-tissue imaging with high spatiotemporal resolution.; National Science Foundation of China [21573013, 21390412, 31271423, 31327901]; 863 Program [SS2015AA020406]; CAS Interdisciplinary Innovation Team; SCI(E); EI; PubMed; ARTICLE; sun_yujie@pku.edu.cn; 19; 9982-9987; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433942]
专题	生命科学学院工学院
推荐引用方式 GB/T 7714	Chen, Xuanze,Zong, Weijian,Li, Rongqin,et al. Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation[J]. NANOSCALE,2016.
APA	Chen, Xuanze.,Zong, Weijian.,Li, Rongqin.,Zeng, Zhiping.,Zhao, Jia.,...&Sun, Yujie.(2016).Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation.NANOSCALE.
MLA	Chen, Xuanze,et al."Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation".NANOSCALE (2016).