RNA topoisomerase is prevalent in all domains of life and associates   with polyribosomes in animals

doi:10.1093/nar/gkw508

CORC > 北京大学 > 生命科学学院

	RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals
	Ahmad, Muzammil ; Xue, Yutong ; Lee, Seung Kyu ; Martindale, Jennifer L. ; Shen, Weiping ; Li, Wen ; Zou, Sige ; Ciaramella, Maria ; Debat, Helene ; Nadal, Marc ; Leng, Fenfei ; Zhang, Hongliang ; Wang, Quan ; Siaw, Grace Ee-Lu ; Niu, Hengyao ; Pommier, Yves ; Gorospe, Myriam ; Hsieh, Tao-Shih ; Tse-Dinh, Yuk-Ching ; Xu, Dongyi ; Wang, Weidong
刊名	NUCLEIC ACIDS RESEARCH
	2016
关键词	X-SYNDROME PROTEIN DNA TOPOISOMERASE ESCHERICHIA-COLI REVERSE GYRASE CRYSTAL-STRUCTURE CELL-LINES COMPLEX BINDING GENOME RELAXATION
DOI	10.1093/nar/gkw508
英文摘要	DNA Topoisomerases are essential to resolve topological problems during DNA metabolism in all species. However, the prevalence and function of RNA topoisomerases remain uncertain. Here, we show that RNA topoisomerase activity is prevalent in Type IA topoisomerases from bacteria, archaea, and eukarya. Moreover, this activity always requires the conserved Type IA core domains and the same catalytic residue used in DNA topoisomerase reaction; however, it does not absolutely require the non-conserved carboxyl- terminal domain (CTD),which is necessary for relaxation reactions of supercoiled DNA. The RNA topoisomerase activity of human Top3 beta differs from that of Escherichia coli topoisomerase I in that the former but not the latter requires the CTD, indicating that topoisomerases have developed distinct mechanisms during evolution to catalyze RNA topoisomerase reactions. Notably, Top3 beta proteins from several animals associate with polyribosomes, which are units of mRNA translation, whereas the Top3 homologs from E. coli and yeast lack the association. The Top3 beta-polyribosome association requires TDRD3, which directly interacts with Top3 beta and is present in animals but not bacteria or yeast. We propose that RNA topoisomerases arose in the early RNA world, and that they are retained through all domains of DNA-based life, where they mediate mRNA translation as part of polyribosomes in animals.; Intramural Research Program of the National Institute on Aging [Z01 AG000657-08]; National Cancer Institute [Z01 BC006161]; National Institutes of Health; National Basic Research Program of China [2013CB911002]; National Natural Science Foundation of China [31271435]; National Institute on Aging, NIH, Intramural funding; SCI(E); PubMed; ARTICLE; xudongyi@pku.edu.cn; wangw@grc.nia.nih.gov; 13; 6335-6349; 44
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433483]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ahmad, Muzammil,Xue, Yutong,Lee, Seung Kyu,et al. RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals[J]. NUCLEIC ACIDS RESEARCH,2016.
APA	Ahmad, Muzammil.,Xue, Yutong.,Lee, Seung Kyu.,Martindale, Jennifer L..,Shen, Weiping.,...&Wang, Weidong.(2016).RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals.NUCLEIC ACIDS RESEARCH.
MLA	Ahmad, Muzammil,et al."RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals".NUCLEIC ACIDS RESEARCH (2016).