Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR   KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot   Regeneration In Vitro

doi:10.1007/s11105-014-0769-2

CORC > 北京大学 > 生命科学学院

	Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro
	Li, Xia ; Fang, Yu-Han ; Han, Jing-Dan ; Bai, Shu-Nong ; Rao, Guang-Yuan
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2015
关键词	Adiantum capillus-veneris Gene expression AcvSERK1 gene Shoot regeneration Homolog LEUCINE-RICH REPEAT PLATYCERIUM-CORONARIUM SERK GENE PLANT DEFENSE EMBRYOS CULTURES CALLUS FAMILY CELL
DOI	10.1007/s11105-014-0769-2
英文摘要	SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes are expressed primarily in seed plants and are involved in the development of embryos from already-differentiated plant cells. This study provides the first evidence for the existence of a SERK gene, designated AcvSERK1, in a fern, Adiantum capillus-veneris. AcvSERK1 belongs to a small family of receptor-like kinases (RLKs), and its sequence and conserved structure closely resemble SERK homologs in other land plants. Phylogenetic analysis of the SERK gene family showed that AcvSERK1 forms a clade with SERK-like genes from non-seed plants and is a member of the SERK1/2 genes group, involved in somatic embryogenesis. Moreover, our analyses reveal that AcvSERK1 is expressed throughout the entire process of embryo development, but its levels decrease with the formation of shoots and seedlings. Additionally, in situ hybridization showed that AcvSERK1 is expressed during green globular body (GGB)-derived somatic embryogenesis and in meristematic calluses. Our results indicate that the AcvSERK1 gene is a good marker for differentiated cells that are capable of forming embryos in A. capillus-veneris, suggesting that SERK genes have played an important role in embryogenesis during the evolution of land plants.; National Natural Science Foundation of China (NSFC) [91231105]; SCI(E); ARTICLE; rao@pku.edu.cn; 3; 638-647; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/419776]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Xia,Fang, Yu-Han,Han, Jing-Dan,et al. Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015.
APA	Li, Xia,Fang, Yu-Han,Han, Jing-Dan,Bai, Shu-Nong,&Rao, Guang-Yuan.(2015).Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro.PLANT MOLECULAR BIOLOGY REPORTER.
MLA	Li, Xia,et al."Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro".PLANT MOLECULAR BIOLOGY REPORTER (2015).