A DNA ligase required for active DNA demethylation and genomic   imprinting in Arabidopsis

doi:10.1038/cr.2015.45

CORC > 北京大学 > 生命科学学院

	A DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis
	Li, Yan ; Duan, Cheng-Guo ; Zhu, Xiaohong ; Qian, Weiqiang ; Zhu, Jian-Kang
刊名	CELL RESEARCH
	2015
关键词	REPAIR GENE THALIANA ENDOSPERM HOMOLOG DEMETER PROTEIN
DOI	10.1038/cr.2015.45
英文摘要	Active DNA demethylation plays crucial roles in the regulation of gene expression and gene imprinting. In plants, active DNA demethylation is initiated by the ROS1/DME family of 5-methylcytosine-specific DNA glycosylases via a base excision repair mechanism [1, 2]. ROS1 and DME are bifunctional DNA glycosylases that excise the 5-methylcytosine base and then cleave the DNA backbone at the abasic site, resulting in a gap with a 3' phosphate or 3' dRP (3' α, β-unsaturated aldehyde)terminus. The DNA phosphatase ZDP and the apurinic/ apyrimidinic endonuclease APE1L process the 3' phosphate and 3' dRP termini, respectively, to generate a 3' OH group so that downstream polymerases and ligases can fill in the gap with an unmethylated cytosine [3, 4]. Thus far, the DNA polymerases and ligases involved in this active DNA demethylation pathway are unclear. Here we show that Arabidopsis DNA LIGASE I (AtLIGl) co-localizes with ROS1, ZDP and APE1L in vivo. In addition, we found that AtLIGl is essential for demethylation and activation of the maternally imprinted genes FWA and MEA in the endosperm. Our data suggest that AtLIGl is the major DNA ligase that functions at the last step in active DNA demethylation in Arabidopsis.; by the Chinese Academy of Sciences and by Peking-Tsinghua Center for Life Science,supported by US National Institutes of Health Grants; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); LETTER; jkzhu@purdue.edu; 6; 757-760; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/419704]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, Yan,Duan, Cheng-Guo,Zhu, Xiaohong,et al. A DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis[J]. CELL RESEARCH,2015.
APA	Li, Yan,Duan, Cheng-Guo,Zhu, Xiaohong,Qian, Weiqiang,&Zhu, Jian-Kang.(2015).A DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis.CELL RESEARCH.
MLA	Li, Yan,et al."A DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis".CELL RESEARCH (2015).