Structures of the RNA polymerase-sigma(54) reveal new and conserved   regulatory strategies

doi:10.1126/science.aab1478

CORC > 北京大学 > 生命科学学院

	Structures of the RNA polymerase-sigma(54) reveal new and conserved regulatory strategies
	Yang, Yun ; Darbari, Vidya C. ; Zhang, Nan ; Lu, Duo ; Glyde, Robert ; Wang, Yi-Ping ; Winkelman, Jared T. ; Gourse, Richard L. ; Murakami, Katsuhiko S. ; Buck, Martin ; Zhang, Xiaodong
刊名	SCIENCE
	2015
关键词	II-TFIIB COMPLEX TRANSCRIPTION INITIATION ESCHERICHIA-COLI SINGLE-MOLECULE BACTERIAL SIGMA DNA POLYMERASES DOMAIN HOLOENZYME
DOI	10.1126/science.aab1478
英文摘要	Transcription by RNA polymerase (RNAP) in bacteria requires specific promoter recognition by sigma factors. The major variant s factor (sigma(54)) initially forms a transcriptionally silent complex requiring specialized adenosine triphosphate-dependent activators for initiation. Our crystal structure of the 450-kilodalton RNAP-sigma(54) holoenzyme at 3.8 angstroms reveals molecular details of sigma(54) and its interactions with RNAP. The structure explains how sigma(54) targets different regions in RNAP to exert its inhibitory function. Although sigma(54) and the major s factor, sigma(70), have similar functional domains and contact similar regions of RNAP, unanticipated differences are observed in their domain arrangement and interactions with RNAP, explaining their distinct properties. Furthermore, we observe evolutionarily conserved regulatory hotspots in RNAPs that can be targeted by a diverse range of mechanisms to fine tune transcription.; Chinese National Science Foundation; China Scholarship Council; UK Biotechnology and Biological Sciences Research Council; NIH [GM087350, R37 GM37048]; National Key Basic Research Programme in China [2015CB755700]; SCI(E); PubMed; ARTICLE; xiaodong.zhang@imperial.ac.uk; 6250; 882-885; 349
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417116]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, Yun,Darbari, Vidya C.,Zhang, Nan,et al. Structures of the RNA polymerase-sigma(54) reveal new and conserved regulatory strategies[J]. SCIENCE,2015.
APA	Yang, Yun.,Darbari, Vidya C..,Zhang, Nan.,Lu, Duo.,Glyde, Robert.,...&Zhang, Xiaodong.(2015).Structures of the RNA polymerase-sigma(54) reveal new and conserved regulatory strategies.SCIENCE.
MLA	Yang, Yun,et al."Structures of the RNA polymerase-sigma(54) reveal new and conserved regulatory strategies".SCIENCE (2015).