Unique conformer selection of human growth-regulatory lectin galectin-1   for ganglioside GM(1) versus bacterial toxins

doi:10.1021/bi035477c

CORC > 北京大学 > 生命科学学院

	Unique conformer selection of human growth-regulatory lectin galectin-1 for ganglioside GM(1) versus bacterial toxins
	Siebert, HC ; Andre, S ; Lu, SY ; Frank, M ; Kaltner, H ; van Kuik, JA ; Korchagina, EY ; Bovin, N ; Tajkhorshid, E ; Kaptein, R ; Vliegenthart, JFG ; von der Lieth, CW ; Jimenez-Barbero, J ; Kopitz, J ; Gabius, HJ
刊名	biochemistry us
	2003
关键词	HUMAN NEUROBLASTOMA-CELLS MOLECULAR-DYNAMICS SIMULATIONS HEAT-LABILE ENTEROTOXIN CHOLERA-TOXIN MISTLETOE LECTIN LIGAND-BINDING SIALIC-ACID AFFINITY-CHROMATOGRAPHY OLIGOSACCHARIDE CHAIN CARBOHYDRATE-BINDING
DOI	10.1021/bi035477c
英文摘要	Endogenous lectins induce effects on cell growth by binding to antennae of natural glycoconjugates. These complex carbohydrates often present more than one potential lectin-binding site in a single chain. Using the growth-regulatory interaction of the pentasaccharide of ganglioside GM, with homodimeric galectin-1 on neuroblastoma cell surfaces as a model, we present a suitable strategy for addressing this issue. The approach combines NMR spectroscopic and computational methods and does not require isotope-labeled glycans. It involves conformational analysis of the two building blocks of the GM(1) glycan, i.e., the disaccharide Galbeta1-3GalNAc and the trisaccharide Neu5Acalpha2-3Galbeta1-4Glc. Their bound-state conformations were determined by transferred nuclear Overhauser enhancement spectroscopy. Next, measurements on the lectin-pentasaccharide complex revealed differential conformer selection regarding the sialylgalactose linkage in the tri- versus pentasaccharide (Phi and psi value of -70degrees and 15degrees vs 70degrees and 15degrees, respectively). To proceed in the structural analysis, the characteristic experimentally detected spatial vicinity of a galactose unit and Trp68 in the galectin's binding site offered a means, exploiting saturation transfer from protein to carbohydrate protons. Indeed, we detected two signals unambiguously assigned to the terminal Gal and the GalNAc residues. Computational docking and interaction energy analyses of the entire set of ligands supported and added to experimental results. The finding that the ganglioside's carbohydrate chain is subject to differential conformer selection at the sialylgalactose linkage by galectin-1 and GM(1)-binding cholera toxin ((D and IF values of -172degrees and -26degrees, respectively) is relevant for toxin-directed drug design. In principle, our methodology can be applied in studies aimed at blocking galectin functionality in malignancy and beyond glycosciences.; Biochemistry & Molecular Biology; SCI(E); EI; 94; ARTICLE; 50; 14762-14773; 42
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/401724]
专题	生命科学学院
推荐引用方式 GB/T 7714	Siebert, HC,Andre, S,Lu, SY,et al. Unique conformer selection of human growth-regulatory lectin galectin-1 for ganglioside GM(1) versus bacterial toxins[J]. biochemistry us,2003.
APA	Siebert, HC.,Andre, S.,Lu, SY.,Frank, M.,Kaltner, H.,...&Gabius, HJ.(2003).Unique conformer selection of human growth-regulatory lectin galectin-1 for ganglioside GM(1) versus bacterial toxins.biochemistry us.
MLA	Siebert, HC,et al."Unique conformer selection of human growth-regulatory lectin galectin-1 for ganglioside GM(1) versus bacterial toxins".biochemistry us (2003).