A novel peptide isolated from a phage display peptide library with   trastuzumab can mimic antigen epitope of HER-2

doi:10.1074/jcb.M411047200

CORC > 北京大学 > 生命科学学院

	A novel peptide isolated from a phage display peptide library with trastuzumab can mimic antigen epitope of HER-2
	Jiang, BH ; Liu, WB ; Qu, H ; Meng, L ; Song, SM ; Tao, OY ; Shou, CC
刊名	journal of biological chemistry
	2005
关键词	ERBB SIGNALING NETWORK BREAST-CANCER NEU ONCOGENE VACCINE PROTEIN RECEPTOR CELL HERCEPTIN ANTIBODY
DOI	10.1074/jcb.M411047200
英文摘要	Trastuzumab, a humanized antibody to HER-2, has been shown to be effective in the treatment of breast cancer in which HER-2 overexpression and metastasis occurs. In our search for an effective mimic epitope of HER-2 binding with trastuzumab and to develop HER-2 peptide vaccine, we screened a phage display 12-mer peptide library with trastuzumab as the target. A mimetic peptide (mimotope) H98 (LLGPYELWELSH) that could specifically recognize trastuzumab was isolated. The DNA encoding peptide H98 was cloned and expressed as the fusion protein GST-H98 in Escherichia coli BL21. The purified GST-H98 could specifically bind to trastuzumab and block the binding of trastuzumab to HER-2 protein. Moreover, H98 could significantly block the function of trastuzumab inhibiting the growth of cancer cells. Mice that were immunized with GST-H98 made specific antibody to H98 as well as to HER-2. In addition, T-cell proliferation occurred in mice immunized with GST-H98. Although no sequence homology was found between H98 and HER-2, through the use of structure analysis we were able to determine that peptide H98 contributed to a conformational epitope of HER-2. Furthermore, we determined that the last two amino acids at the C terminus, and the third together with the fourth amino acid at the N terminus of peptide H98 are critical to the binding of H98 to trastuzumab. As a result, we conclude that peptide H98 has potential for being developed as a HER-2 vaccine for biotherapy of cancer with HER-2 overexpression.; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 27; ARTICLE; 6; 4656-4662; 280
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/399893]
专题	生命科学学院
推荐引用方式 GB/T 7714	Jiang, BH,Liu, WB,Qu, H,et al. A novel peptide isolated from a phage display peptide library with trastuzumab can mimic antigen epitope of HER-2[J]. journal of biological chemistry,2005.
APA	Jiang, BH.,Liu, WB.,Qu, H.,Meng, L.,Song, SM.,...&Shou, CC.(2005).A novel peptide isolated from a phage display peptide library with trastuzumab can mimic antigen epitope of HER-2.journal of biological chemistry.
MLA	Jiang, BH,et al."A novel peptide isolated from a phage display peptide library with trastuzumab can mimic antigen epitope of HER-2".journal of biological chemistry (2005).