ETHYLENE-INSENSITIVE5 encodes a 5 &apos;-&gt; 3 &apos; exoribonuclease required for   regulation of the EIN3-targeting F-box proteins EBF1/2

doi:10.1073/pnas.0605528103

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	ETHYLENE-INSENSITIVE5 encodes a 5 '-> 3 ' exoribonuclease required for regulation of the EIN3-targeting F-box proteins EBF1/2
	Olmedo, Gabriela ; Guo, Hongwei ; Gregory, Brian D. ; Nourizadeh, Saeid D. ; Aguilar-Henonin, Laura ; Li, Hongjiang ; An, Fengying ; Guzman, Plinio ; Ecker, Joseph R.
刊名	proceedings of the national academy of sciences of the united states of america
	2006
关键词	Arabidopsis growth regulation signal transduction ETHYLENE SIGNAL-TRANSDUCTION OF-FUNCTION MUTATION RESPONSE PATHWAY ARABIDOPSIS-THALIANA MESSENGER-RNAS GENOME MUTANT GENE GAS EXPRESSION
DOI	10.1073/pnas.0605528103
英文摘要	Ethylene is a gaseous plant growth regulator that controls a multitude of developmental and stress responses. Recently, the levels of Arabidopsis EIN3 protein, a key transcription factor mediating ethylene-regulated gene expression, have been demonstrated to increase in response to the presence of ethylene gas. Furthermore, in the absence of ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F-box proteins, EBF1 and EBF2. Here we report the identification of ETHYLENE-INSENSITIVE5 as the 5'-> 3' exoribonuclease XRN4. Specifically, we demonstrate that EIN5 is a component of the ethylene signal transduction cascade acting downstream of CTR1 that is required for ethylene-mediated gene expression changes. Furthermore, we find that the ethylene insensitivity of ein5 mutant plants is a consequence of the over-accumulation of EBF1 and EBF2 mRNAs resulting in the under-accumulation of EIN3 even in the presence of ethylene gas. Together, our results suggest that the role of EIN5 in ethylene perception is to antagonize the negative feedback regulation on EIN3 by promoting EBF1 and EBF2 mRNA decay, which consequently allows the accumulation of EIN3 protein to trigger the ethylene response.; Multidisciplinary Sciences; SCI(E); 90; ARTICLE; 36; 13286-13293; 103
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/398362]
专题	生命科学学院
推荐引用方式 GB/T 7714	Olmedo, Gabriela,Guo, Hongwei,Gregory, Brian D.,et al. ETHYLENE-INSENSITIVE5 encodes a 5 '-> 3 ' exoribonuclease required for regulation of the EIN3-targeting F-box proteins EBF1/2[J]. proceedings of the national academy of sciences of the united states of america,2006.
APA	Olmedo, Gabriela.,Guo, Hongwei.,Gregory, Brian D..,Nourizadeh, Saeid D..,Aguilar-Henonin, Laura.,...&Ecker, Joseph R..(2006).ETHYLENE-INSENSITIVE5 encodes a 5 '-> 3 ' exoribonuclease required for regulation of the EIN3-targeting F-box proteins EBF1/2.proceedings of the national academy of sciences of the united states of america.
MLA	Olmedo, Gabriela,et al."ETHYLENE-INSENSITIVE5 encodes a 5 '-> 3 ' exoribonuclease required for regulation of the EIN3-targeting F-box proteins EBF1/2".proceedings of the national academy of sciences of the united states of america (2006).