Fluorescent Protein Engineering Through Genetic Incorporation of   3-Chlorotyrosine

doi:10.3724/SP.J.1206.2011.00299

CORC > 北京大学 > 生命科学学院

	Fluorescent Protein Engineering Through Genetic Incorporation of 3-Chlorotyrosine
	Zhang Wei ; Wang Yue-Qi ; Wang Jiang-Yun
刊名	生物化学与生物物理进展
	2012
关键词	GFP unnatural amino acid genetic code expansion APOLIPOPROTEIN-A-I MYELOPEROXIDASE CHLORINATION SITE
DOI	10.3724/SP.J.1206.2011.00299
英文摘要	Posttranslational chlorination of tyrosine residues in proteins produce 3-chlorotyrosine (3-Cl-Tyr), which is associated with several diseases, including Alzheimer's disease, asthma, atherosclerosis and acute myocardial infarction. High level of 3-chlorotyrosine has been found in ApoA1 protein in atherosclerosis patients, indicating that it may play important role in disease. Here we report a new method to facilitate the site-specific incorporation of 3-chlorotyrosine into proteins at specific sites. Such a new method may be very useful to probe the regulatory role of tyrosine chlorination in protein function. Compared to tyrosine (Tyr), 3-Cl-Tyr has lower pK(a). We replaced the green fluorescent protein (GFP) and photoactivatable protein mEOS2 chromophore Tyr (Tyr66 in GFP) by 3-Cl-Tyr, lowering the chromophore pK(a) to 4.2 and 4.7, respectively. These mutant fluorescent proteins with lower pK(a) may be advantageous for labeling proteins in acidic organelles such as lysosome and phagosome.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 4; 378-387; 39
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/393498]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang Wei,Wang Yue-Qi,Wang Jiang-Yun. Fluorescent Protein Engineering Through Genetic Incorporation of 3-Chlorotyrosine[J]. 生物化学与生物物理进展,2012.
APA	Zhang Wei,Wang Yue-Qi,&Wang Jiang-Yun.(2012).Fluorescent Protein Engineering Through Genetic Incorporation of 3-Chlorotyrosine.生物化学与生物物理进展.
MLA	Zhang Wei,et al."Fluorescent Protein Engineering Through Genetic Incorporation of 3-Chlorotyrosine".生物化学与生物物理进展 (2012).