Fruit-specific RNAi-mediated suppression of SlNCED1 increases both   lycopene and beta-carotene contents in tomato fruit

doi:10.1093/jxb/ers026

CORC > 北京大学 > 生命科学学院

	Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and beta-carotene contents in tomato fruit
	Sun, Liang ; Yuan, Bing ; Zhang, Mei ; Wang, Ling ; Cui, Mengmeng ; Wang, Qi ; Leng, Ping
刊名	journal of experimental botany
	2012
关键词	ABA beta-carotene carotenoid biosynthesis ethylene fruit ripening lycopene SlNCED1-RNAi tomato ABSCISIC-ACID BIOSYNTHESIS LYCOPERSICON-ESCULENTUM ZEAXANTHIN EPOXIDASE ETHYLENE PRODUCTION FLAVONOID CONTENT PLANT DEVELOPMENT ANTISENSE RNA EXPRESSION PROMOTER ABA
DOI	10.1093/jxb/ers026
英文摘要	Abscisic acid (ABA) plays important roles during tomato fruit ripening. To study the regulation of carotenoid biosynthesis by ABA, the SlNCED1 gene encoding 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the ABA biosynthesis, was suppressed in tomato plants by transformation with an RNA interference (RNAi) construct driven by a fruit-specific E8 promoter. ABA accumulation and SlNCED1 transcript levels in the transgenic fruit were down-regulated to between 20-50% of that in control fruit. This significant reduction in NCED activity led to the carbon that normally channels to free ABA as well as the ABA metabolite accumulation during ripening to be partially blocked. Therefore, this 'backlogged' carbon transformed into the carotenoid pathway in the RNAi lines resulted in increased assimilation and accumulation of upstream compounds in the pathway, chiefly lycopene and beta-carotene. Fruit of all RNAi lines displayed deep red coloration compared with the pink colour of control fruit. The decrease in endogenous ABA in these transgenics resulted in an increase in ethylene, by increasing the transcription of genes related to the synthesis of ethylene during ripening. In conclusion, ABA potentially regulated the degree of pigmentation and carotenoid composition during ripening and could control, at least in part, ethylene production and action in climacteric tomato fruit.; Plant Sciences; SCI(E); 0; ARTICLE; 8; 3097-3108; 63
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/393453]
专题	生命科学学院
推荐引用方式 GB/T 7714	Sun, Liang,Yuan, Bing,Zhang, Mei,et al. Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and beta-carotene contents in tomato fruit[J]. journal of experimental botany,2012.
APA	Sun, Liang.,Yuan, Bing.,Zhang, Mei.,Wang, Ling.,Cui, Mengmeng.,...&Leng, Ping.(2012).Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and beta-carotene contents in tomato fruit.journal of experimental botany.
MLA	Sun, Liang,et al."Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and beta-carotene contents in tomato fruit".journal of experimental botany (2012).