DEXH Box RNA Helicase-Mediated Mitochondrial Reactive Oxygen Species   Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and   Auxin Signaling

doi:10.1105/tpc.112.098707

CORC > 北京大学 > 生命科学学院

	DEXH Box RNA Helicase-Mediated Mitochondrial Reactive Oxygen Species Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and Auxin Signaling
	He, Junna ; Duan, Ying ; Hua, Deping ; Fan, Guangjiang ; Wang, Li ; Liu, Yue ; Chen, Zhizhong ; Han, Lihua ; Qu, Li-Jia ; Gong, Zhizhong
刊名	plant cell
	2012
关键词	PROTEIN PHOSPHATASE 2C HYDROGEN-PEROXIDE STRESS RESPONSES ROOT DEVELOPMENT COMPLEX-I RESPIRATORY-CHAIN PLANT DEVELOPMENT OXIDATIVE STRESS GUARD-CELLS THALIANA
DOI	10.1105/tpc.112.098707
英文摘要	It is well known that abscisic acid (ABA) promotes reactive oxygen species (ROS) production through plasma membrane-associated NADPH oxidases during ABA signaling. However, whether ROS from organelles can act as second messengers in ABA signaling is largely unknown. Here, we identified an ABA overly sensitive mutant, abo6, in a genetic screen for ABA-mediated inhibition of primary root growth. ABO6 encodes a DEXH box RNA helicase that is involved in regulating the splicing of several genes of complex I in mitochondria. The abo6 mutant accumulated more ROS in mitochondria, as established using a mitochondrial superoxide indicator, circularly permuted yellow fluorescent protein. Two dominant-negative mutations in ABA insensitive1 (abi1-1) and abi2-1 greatly reduced ROS production in mitochondria. The ABA sensitivity of abo6 can also be compromised by the atrbohF mutation. ABA-mediated inhibition of seed germination and primary root growth in abo6 was released by the addition of reduced GSH and exogenous auxin to the medium. Expression of auxin-responsive markers ProDR5:GUS (for synthetic auxin response element D1-4 with site-directed mutants in the 5'-end from soybean): beta-glucuronidase) and Indole-3-acetic acid inducible2:GUS was greatly reduced by the abo6 mutation. Hence, our results provide molecular evidence for the interplay between ABA and auxin through the production of ROS from mitochondria. This interplay regulates primary root growth and seed germination in Arabidopsis thaliana.; Biochemistry & Molecular Biology; Plant Sciences; Cell Biology; SCI(E); 0; ARTICLE; 5; 1815-1833; 24
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/393430]
专题	生命科学学院
推荐引用方式 GB/T 7714	He, Junna,Duan, Ying,Hua, Deping,et al. DEXH Box RNA Helicase-Mediated Mitochondrial Reactive Oxygen Species Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and Auxin Signaling[J]. plant cell,2012.
APA	He, Junna.,Duan, Ying.,Hua, Deping.,Fan, Guangjiang.,Wang, Li.,...&Gong, Zhizhong.(2012).DEXH Box RNA Helicase-Mediated Mitochondrial Reactive Oxygen Species Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and Auxin Signaling.plant cell.
MLA	He, Junna,et al."DEXH Box RNA Helicase-Mediated Mitochondrial Reactive Oxygen Species Production in Arabidopsis Mediates Crosstalk between Abscisic Acid and Auxin Signaling".plant cell (2012).