A large-scale zebrafish gene knockout resource for the genome-wide study   of gene function

doi:10.1101/gr.151464.112

CORC > 北京大学 > 生命科学学院

	A large-scale zebrafish gene knockout resource for the genome-wide study of gene function
	Varshney, Gaurav K. ; Lu, Jing ; Gildea, Derek E. ; Huang, Haigen ; Pei, Wuhong ; Yang, Zhongan ; Huang, Sunny C. ; Schoenfeld, David ; Pho, Nam H. ; Casero, David ; Hirase, Takashi ; Mosbrook-Davis, Deborah ; Zhang, Suiyuan ; Jao, Li-En ; Zhang, Bo ; Woods, Ian G. ; Zimmerman, Steven ; Schier, Alexander F. ; Wolfsberg, Tyra G. ; Pellegrini, Matteo ; Burgess, Shawn M. ; Lin, Shuo
刊名	genome research
	2013
关键词	INSERTIONAL MUTAGENESIS POSITIONAL CLONING INTEGRATION RETROVIRUS REGIONS EFFICIENCY MUTATIONS TARGETS
DOI	10.1101/gr.151464.112
英文摘要	With the completion of the zebrafish genome sequencing project, it becomes possible to analyze the function of zebrafish genes in a systematic way. The first step in such an analysis is to inactivate each protein-coding gene by targeted or random mutation. Here we describe a streamlined pipeline using proviral insertions coupled with high-throughput sequencing and mapping technologies to widely mutagenize genes in the zebrafish genome. We also report the first 6144 mutagenized and archived Firs predicted to carry up to 3776 mutations in annotated genes. Using in vitro fertilization, we have rescued and characterized similar to 0.5% of the predicted mutations, showing mutation efficacy and a variety of phenotypes relevant to both developmental processes and human genetic diseases. Mutagenized fish lines are being made freely available to the public through the Zebrafish International Resource Center. These fish lines establish an important milestone for zebrafish genetics research and should greatly facilitate systematic functional studies of the vertebrate genome.; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; SCI(E); 16; ARTICLE; 4; 727-735; 23
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/391748]
专题	生命科学学院
推荐引用方式 GB/T 7714	Varshney, Gaurav K.,Lu, Jing,Gildea, Derek E.,et al. A large-scale zebrafish gene knockout resource for the genome-wide study of gene function[J]. genome research,2013.
APA	Varshney, Gaurav K..,Lu, Jing.,Gildea, Derek E..,Huang, Haigen.,Pei, Wuhong.,...&Lin, Shuo.(2013).A large-scale zebrafish gene knockout resource for the genome-wide study of gene function.genome research.
MLA	Varshney, Gaurav K.,et al."A large-scale zebrafish gene knockout resource for the genome-wide study of gene function".genome research (2013).