Telomere Reprogramming and Maintenance in Porcine iPS Cells

doi:10.1371/journal.pone.0074202

CORC > 北京大学 > 生命科学学院

	Telomere Reprogramming and Maintenance in Porcine iPS Cells
	Ji, Guangzhen ; Ruan, Weimin ; Liu, Kai ; Wang, Fang ; Sakellariou, Despoina ; Chen, Jijun ; Yang, Yang ; Okuka, Maja ; Han, Jianyong ; Liu, Zhonghua ; Lai, Liangxue ; Gagos, Sarantis ; Xiao, Lei ; Deng, Hongkui ; Li, Ning ; Liu, Lin
刊名	plos one
	2013
关键词	PLURIPOTENT STEM-CELLS MAMMALIAN TELOMERES GENOMIC STABILITY SOMATIC-CELLS MINIATURE PIG GENERATION LENGTH CIRCLES MOUSE DNA
DOI	10.1371/journal.pone.0074202
英文摘要	Telomere reprogramming and silencing of exogenous genes have been demonstrated in mouse and human induced pluripotent stem cells (iPS cells). Pigs have the potential to provide xenotransplant for humans, and to model and test human diseases. We investigated the telomere length and maintenance in porcine iPS cells generated and cultured under various conditions. Telomere lengths vary among different porcine iPS cell lines, some with telomere elongation and maintenance, and others telomere shortening. Porcine iPS cells with sufficient telomere length maintenance show the ability to differentiate in vivo by teratoma formation test. IPS cells with short or dysfunctional telomeres exhibit reduced ability to form teratomas. Moreover, insufficient telomerase and incomplete telomere reprogramming and/or maintenance link to sustained activation of exogenous genes in porcine iPS cells. In contrast, porcine iPS cells with reduced expression of exogenous genes or partial exogene silencing exhibit insufficient activation of endogenous pluripotent genes and telomerase genes, accompanied by telomere shortening with increasing passages. Moreover, telomere doublets, telomere sister chromatid exchanges and t-circles that presumably are involved in telomere lengthening by recombination also are found in porcine iPS cells. These data suggest that both telomerase-dependent and telomerase-independent mechanisms are involved in telomere reprogramming during induction and passages of porcine iPS cells, but these are insufficient, resulting in increased telomere damage and shortening, and chromosomal instability. Active exogenes might compensate for insufficient activation of endogenous genes and incomplete telomere reprogramming and maintenance of porcine iPS cells. Further understanding of telomere reprogramming and maintenance may help improve the quality of porcine iPS cells.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000325423500009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 10; ARTICLE; 9; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/391089]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ji, Guangzhen,Ruan, Weimin,Liu, Kai,et al. Telomere Reprogramming and Maintenance in Porcine iPS Cells[J]. plos one,2013.
APA	Ji, Guangzhen.,Ruan, Weimin.,Liu, Kai.,Wang, Fang.,Sakellariou, Despoina.,...&Liu, Lin.(2013).Telomere Reprogramming and Maintenance in Porcine iPS Cells.plos one.
MLA	Ji, Guangzhen,et al."Telomere Reprogramming and Maintenance in Porcine iPS Cells".plos one (2013).