Regulation of DNA methylation turnover at LTR retrotransposons and   imprinted loci by the histone methyltransferase Setdb1

doi:10.1073/pnas.1322273111

CORC > 北京大学 > 生命科学学院

	Regulation of DNA methylation turnover at LTR retrotransposons and imprinted loci by the histone methyltransferase Setdb1
	Leung, Danny ; Du, Tingting ; Wagner, Ulrich ; Xie, Wei ; Lee, Ah Young ; Goyal, Preeti ; Li, Yujing ; Szulwach, Keith E. ; Jin, Peng ; Lorincz, Matthew C. ; Ren, Bing
刊名	proceedings of the national academy of sciences of the united states of america
	2014
关键词	epigenomics histone modifications repetitive elements 5-hydroxymethylcytosine EMBRYONIC STEM-CELLS PRIMORDIAL GERM-CELLS CONTROL REGIONS MAMMALIAN DNA 5-HYDROXYMETHYLCYTOSINE TET1 5-METHYLCYTOSINE DEMETHYLATION TRANSCRIPTION CONVERSION
DOI	10.1073/pnas.1322273111
英文摘要	During mammalian development, DNA methylation patterns need to be reset in primordial germ cells (PGCs) and preimplantation embryos. However, many LTR retrotransposons and imprinted genes are impervious to such global epigenetic reprogramming via hitherto undefined mechanisms. Here, we report that a subset of such genomic regions are resistant to widespread erasure of DNA methylation in mouse embryonic stem cells (mESCs) lacking the de novo DNA methyltransferases (Dnmts) Dnmt3a and Dnmt3b. Intriguingly, these loci are enriched for H3K9me3 in mESCs, implicating this mark in DNA methylation homeostasis. Indeed, deletion of the H3K9 methyltransferase SET domain bifurcated 1 (Setdb1) results in reduced H3K9me3 and DNA methylation levels at specific loci, concomitant with increased 5-hydroxymethylation (5hmC) and ten-eleven translocation 1 binding. Taken together, these data reveal that Setdb1 promotes the persistence of DNA methylation in mESCs, likely reflecting one mechanism by which DNA methylation is maintained at LTR retrotransposons and imprinted genes during developmental stages when DNA methylation is reprogrammed.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000335477300049&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 29; ARTICLE; mlorincz@mail.ubc.ca; biren@ucsd.edu; 18; 6690-6695; 111
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389340]
专题	生命科学学院
推荐引用方式 GB/T 7714	Leung, Danny,Du, Tingting,Wagner, Ulrich,et al. Regulation of DNA methylation turnover at LTR retrotransposons and imprinted loci by the histone methyltransferase Setdb1[J]. proceedings of the national academy of sciences of the united states of america,2014.
APA	Leung, Danny.,Du, Tingting.,Wagner, Ulrich.,Xie, Wei.,Lee, Ah Young.,...&Ren, Bing.(2014).Regulation of DNA methylation turnover at LTR retrotransposons and imprinted loci by the histone methyltransferase Setdb1.proceedings of the national academy of sciences of the united states of america.
MLA	Leung, Danny,et al."Regulation of DNA methylation turnover at LTR retrotransposons and imprinted loci by the histone methyltransferase Setdb1".proceedings of the national academy of sciences of the united states of america (2014).