Isolation and identification of proteins with anti-tumor and fibrinolysogen kinase activities from Eisenia foetida

CORC > 北京大学 > 生命科学学院

	Isolation and identification of proteins with anti-tumor and fibrinolysogen kinase activities from Eisenia foetida
	Zhao Rui ; Ji Jian-Guo ; Tong Yuan-Peng ; Chen Qian ; Pu Hai ; Ru Bing-Gen
刊名	sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica
	2002
英文摘要	Proteins from Eisenia foetida possess many biological activities. A group of proteins precipitated by ethanol were isolated and purified by Sephadex G-75 and HiPrep 16/60 DEAE columns, then identified by one- or two- dimensional electrophoresis and mass spectrometry. 2D gel experiments displayed that the pI of proteins from Eisenia foetida were mainly from 3.0 to 4.0. Anti-tumor and kinase activities were determined by in vitro experiments. The enthanol fraction D2(8) showed both of the activities. These ethanol-precipitated proteins were identified further by native polyacrylamide electrophoresis, the protein spots were cut off from gels and digested by trypsin, the peptide mass fingerprints (PMFs) were determined by mass spectrometry. PMF, molecular weight, amino acid composition and N-terminus of 6 proteins were characterized, and band 9 was identified as D2(8). The results suggested that there exist proteins in Eisenia foetida possessed both anti-tumor and fibrinolysogen kinase activities. These methods can be used for identification of the natural bioactive proteins.; SCI(E); PubMed; 0; ARTICLE; 5; 576-82; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/347297]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhao Rui,Ji Jian-Guo,Tong Yuan-Peng,et al. Isolation and identification of proteins with anti-tumor and fibrinolysogen kinase activities from Eisenia foetida[J]. sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica,2002.
APA	Zhao Rui,Ji Jian-Guo,Tong Yuan-Peng,Chen Qian,Pu Hai,&Ru Bing-Gen.(2002).Isolation and identification of proteins with anti-tumor and fibrinolysogen kinase activities from Eisenia foetida.sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica.
MLA	Zhao Rui,et al."Isolation and identification of proteins with anti-tumor and fibrinolysogen kinase activities from Eisenia foetida".sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica (2002).