Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis

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	Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis
	Pan Yi ; Cai Hong ; Li Shu-Xia ; Tian Xia ; Li Tang ; Zhu Yu-Xian
刊名	sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica
	2003
英文摘要	Three proteins secreted from Mycobacterium tuberculosis, Ag85B, ESAT-6 and MPT63 were selected as antigens for making combined DNA vaccine by immunizing mice. The immune response induced by the vaccine and its protective efficacy were studied. It was demonstrated that when mice were immunized with the combined DNA vaccine, the titer of antibody for Ag85B in serum increased to more than 10(5), but the titers of ESAT-6 and MPT63 specific antibodies were undetectable. After the final immunization, the level of gamma specific for Ag85B, ESAT-6 and MPT63 reached (17.0+/-7.0) u/ml, (6.0+/-0.8) u/ml and (11.9+/-8.0) u/ml, respectively. Mice, that were inoculated with the empty eukaryotic expression vector pJW4303 DNA, produced negligible amounts of antigen-specific INF-gamma. The combined DNA vaccine resulted also in significantly reduced amount of bacteria in the lungs of experimental mice. Microphotographs showed clearly that these lungs were better protected against Mycobacterium tuberculosis challenge than control mice. The combined DNA vaccine reported in this work shed new lights on the prophylactic protection against tuberculosis.; SCI(E); PubMed; 0; ARTICLE; 1; 71-6; 35
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/347242]
专题	生命科学学院
推荐引用方式 GB/T 7714	Pan Yi,Cai Hong,Li Shu-Xia,et al. Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis[J]. sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica,2003.
APA	Pan Yi,Cai Hong,Li Shu-Xia,Tian Xia,Li Tang,&Zhu Yu-Xian.(2003).Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis.sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica.
MLA	Pan Yi,et al."Combined recombinant DNA vaccine results in significant protection against Mycobacterium tuberculosis".sheng wu hua xue yu sheng wu wu li xue bao acta biochimica et biophysica sinica (2003).