A subgroup of MYB transcription factor genes undergoes highly conserved   alternative splicing in Arabidopsis and rice

doi:10.1093/jxb/erj094

CORC > 北京大学 > 生命科学学院

	A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice
	Li, JG ; Li, XJ ; Guo, L ; Lu, F ; Feng, XJ ; He, K ; Wei, LP ; Chen, ZL ; Qu, LJ ; Gu, HY
刊名	journal of experimental botany
	2006
关键词	alternative splicing MYB transcription factors nuclear localization signals non-canonical introns nucleotide repeats U12-DEPENDENT INTRONS MESSENGER-RNAS HIGHER-PLANTS TRANSLATION FAMILY EXPRESSION PROTEIN IDENTIFICATION REPRESSION EFFICIENCY
DOI	10.1093/jxb/erj094
英文摘要	MYB transcription factor genes play important roles in many developmental processes and in various defence responses of plants. Two Arabidopsis R2R3-type MYB genes, AtMYB59 and AtMYB48, were found to undergo similar alternative splicing. Both genes have four distinctively spliced transcripts that encode either MYB-related proteins or R2R3-MYB proteins. An extensive BLAST search of the GenBank database resulted in finding and cloning two rice homologues, both of which were also found to share a similar alternative splicing pattern. In a semi-quantitative study, the expression of one splice variant of AtMYB59 was found to be differentially regulated in treatments with different phytohormones and stresses. GFP fusion protein analysis revealed that both of the two predicted nuclear localization signals (NLSs) in the R3 domain are required for localizing to the nucleus. Promoter-GUS analysis in transgenic plants showed that 5'-UTR is sufficient for the translation initiation of type 3 transcripts (encoding R2R3-MYB proteins), but not for type 2 transcripts (encoding MYB-related proteins). Moreover, a new type of non-canonical intron, with the same nucleotide repeats at the 5' and 3' splice sites, was identified. Thirty-eight Arabidopsis and rice genes were found to have this type of non-canonical intron, most of which undergo alternative splicing. These data suggest that this subgroup of transcription factor genes may be involved in multiple biological processes and may be transcriptionally regulated by alternative splicing.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000236613200005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Plant Sciences; SCI(E); EI; PubMed; 55; ARTICLE; 6; 1263-1273; 57
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346085]
专题	生命科学学院
推荐引用方式 GB/T 7714	Li, JG,Li, XJ,Guo, L,et al. A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice[J]. journal of experimental botany,2006.
APA	Li, JG.,Li, XJ.,Guo, L.,Lu, F.,Feng, XJ.,...&Gu, HY.(2006).A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice.journal of experimental botany.
MLA	Li, JG,et al."A subgroup of MYB transcription factor genes undergoes highly conserved alternative splicing in Arabidopsis and rice".journal of experimental botany (2006).