A catalytic mechanism revealed by the crystal structures of the   imidazolonepropionase from Bacillus subtilis

doi:10.1074/jbc.M607703200

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	A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis
	Yu, Yamei ; Liang, Yu-He ; Brostromer, Erik ; Quan, Jun-Min ; Panjikar, Santosh ; Dong, Yu-Hui ; Su, Xiao-Dong
刊名	journal of biological chemistry
	2006
关键词	HISTIDINE AMMONIA-LYASE COLI CYTOSINE DEAMINASE UROCANIC ACID DEGRADATION CRYSTALLIZATION DICTIONARY METABOLISM ENZYMES CLONING HUT
DOI	10.1074/jbc.M607703200
英文摘要	Imidazolonepropionase (EC 3.5.2.7) catalyzes the third step in the universal histidine degradation pathway, hydrolyzing the carbon-nitrogen bonds in 4-imidazolone-5-propionic acid to yield N-formimino-L-glutamic acid. Here we report the crystal structures of the Bacillus subtilis imidazolonepropionase and its complex at 2.0-A resolution with substrate analog imidazole-4-acetic acid sodium (I4AA). The structure of the native enzyme contains two domains, a TIM (triose-phosphate isomerase) barrel domain with two insertions and a small beta-sandwich domain. The TIM barrel domain is quite similar to the members of the alpha/beta barrel metallo-dependent hydrolase superfamily, especially to Escherichia coli cytosine deaminase. A metal ion was found in the central cavity of the TIM barrel and was tightly coordinated to residues His-80, His-82, His-249, Asp-324, and a water molecule. X-ray fluorescence scan analysis confirmed that the bound metal ion was a zinc ion. An acetate ion, 6 A away from the zinc ion, was also found in the potential active site. In the complex structure with I4AA, a substrate analog, I4AA replaced the acetate ion and contacted with Arg-89, Try-102, Tyr-152, His-185, and Glu-252, further defining and confirming the active site. The detailed structural studies allowed us to propose a zinc-activated nucleophilic attack mechanism for the hydrolysis reaction catalyzed by the enzyme.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000242220800052&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); EI; PubMed; 10; ARTICLE; 48; 36929-36936; 281
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345754]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yu, Yamei,Liang, Yu-He,Brostromer, Erik,et al. A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis[J]. journal of biological chemistry,2006.
APA	Yu, Yamei.,Liang, Yu-He.,Brostromer, Erik.,Quan, Jun-Min.,Panjikar, Santosh.,...&Su, Xiao-Dong.(2006).A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis.journal of biological chemistry.
MLA	Yu, Yamei,et al."A catalytic mechanism revealed by the crystal structures of the imidazolonepropionase from Bacillus subtilis".journal of biological chemistry (2006).