Negative regulation of MDA5-but not RIG-I-mediated innate antiviral   signaling by the dihydroxyacetone kinase

doi:10.1073/pnas.0700544104

CORC > 北京大学 > 生命科学学院

	Negative regulation of MDA5-but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase
	Diao, Feici ; Li, Shu ; Tian, Yang ; Zhang, Min ; Xu, Liang-Guo ; Zhang, Yan ; Wang, Rui-Peng ; Chen, Danying ; Zhai, Zhonghe ; Zhong, Bo ; Tien, Po ; Shu, Hong-Bing
刊名	proceedings of the national academy of sciences of the united states of america
	2007
关键词	DAK type IFN interferon regulatory factor-3 NF-kappa B virus NF-KAPPA-B DOUBLE-STRANDED-RNA IFN-BETA PROMOTER INDUCIBLE GENE-I TRANSCRIPTION FACTORS ADAPTER PROTEIN VIRUS-INFECTION IKK-EPSILON ACTIVATION INDUCTION
DOI	10.1073/pnas.0700544104
英文摘要	Viral infection leads to activation of the transcription factors interferon regulatory factor-3 and NF-kappa B, which collaborate to induce type I IFNs. The RNA helicase proteins RIG-I and MDA5 were recently identified as two cytoplasmic viral RNA sensors that recognize different species of viral RNAs produced during viral replication. In this study, we identified DAK, a functionally unknown dihydroacetone kinase, as a specific MDA5-interacting protein. DAK was associated with MDA5, but not RIG-I, under physiological conditions. Overexpression of DAK inhibited MDA5-but not RIG-I- or TLR3-mediated IFN-beta induction. Overexpression of DAK also inhibited cytoplasmic dsRNA and SeV-induced activation of the IFN-P promoter, whereas knockdown of endogenous DAK by RNAi activated the IFN-beta promoter, and increased cytoplasmic dsRNA- or SeV-triggered activation of the IFN-P promoter. In addition, overexpression of DAK inhibited MDA5- but not RIG-I- mediated antiviral activity, whereas DAK RNAi increased cytoplasmic dsRNA-triggered antiviral activity. These findings suggest that DAK is a physiological suppressor of MDA5 and specifically inhibits MDA5- but not RIG-I-mediated innate antiviral signaling.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248063400036&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 71; ARTICLE; 28; 11706-11711; 104
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345530]
专题	生命科学学院
推荐引用方式 GB/T 7714	Diao, Feici,Li, Shu,Tian, Yang,et al. Negative regulation of MDA5-but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase[J]. proceedings of the national academy of sciences of the united states of america,2007.
APA	Diao, Feici.,Li, Shu.,Tian, Yang.,Zhang, Min.,Xu, Liang-Guo.,...&Shu, Hong-Bing.(2007).Negative regulation of MDA5-but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase.proceedings of the national academy of sciences of the united states of america.
MLA	Diao, Feici,et al."Negative regulation of MDA5-but not RIG-I-mediated innate antiviral signaling by the dihydroxyacetone kinase".proceedings of the national academy of sciences of the united states of america (2007).