Lamin B receptor plays a role in stimulating nuclear envelope production   and targeting membrane vesicles to chromatin during nuclear envelope   assembly through direct interaction with importin beta

doi:10.1242/jcs.03355

CORC > 北京大学 > 生命科学学院

	Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta
	Ma, Yan ; Cai, Shang ; Lv, Quanlong ; Jiang, Qing ; Zhang, Quan ; Sodmergen ; Zhai, Zhonghe ; Zhang, Chuanmao
刊名	journal of cell science
	2007
关键词	nuclear envelope assembly lamin B receptor importin beta Ran GTPase XENOPUS EGG EXTRACTS INNER MEMBRANE PORE COMPLEX RAN GTPASE CHROMODOMAIN PROTEINS LOCALIZATION SIGNALS INTEGRAL PROTEIN STRUCTURAL BASIS BINDING-SITES LBR
DOI	10.1242/jcs.03355
英文摘要	Lamin B receptor (LBR), a chromatin and lamin binding protein in the inner nuclear membrane, has been proposed to play a vital role in nuclear envelope (NE) assembly. But the specific role for LBR in NE assembly remains unknown. In the present study, we show that overexpression of LBR causes membrane overproduction, inducing NE invagination and membrane stack formation, and that these processes require the transmembrane domain of LBR. Biochemical analysis shows that the N-terminal domain of LBR directly interacts with importin beta in a Ran sensitive and importin alpha independent manner. Using an in vitro NE assembly assay, we also demonstrate that blocking full length LBR binding sites on importin beta, by the addition of the LBR N-terminal domain inhibits the recruitment of LBR-containing vesicles to importin beta- or Ran-coated beads to form NE structure. Our results suggest that LBR is recruited to chromatin through direct interaction with importin beta to contribute to the fusion of membrane vesicles and formation of the NE.; Cell Biology; SCI(E); PubMed; 37; ARTICLE; 3; 520-530; 120
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345510]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ma, Yan,Cai, Shang,Lv, Quanlong,et al. Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta[J]. journal of cell science,2007.
APA	Ma, Yan.,Cai, Shang.,Lv, Quanlong.,Jiang, Qing.,Zhang, Quan.,...&Zhang, Chuanmao.(2007).Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta.journal of cell science.
MLA	Ma, Yan,et al."Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta".journal of cell science (2007).