The properties of tumor-initiating cells from a hepatocellular carcinoma   patient&apos;s primary and recurrent tumor

doi:10.1093/carcin/bgp232

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	The properties of tumor-initiating cells from a hepatocellular carcinoma patient's primary and recurrent tumor
	Xu, Xiao-Lan ; Xing, Bao-Cai ; Han, Hai-Bo ; Zhao, Wei ; Hu, Mei-Hao ; Xu, Zuo-Liang ; Li, Ji-You ; Xie, Yong ; Gu, Jun ; Wang, Yu ; Zhang, Zhi-Qian
刊名	carcinogenesis
	2010
关键词	CANCER STEM-CELLS B-VIRUS DNA POLY(ADP-RIBOSE) POLYMERASE LIVER-CANCER ALDEHYDE DEHYDROGENASE STEM/PROGENITOR CELLS GENE-EXPRESSION SIDE POPULATION INHIBITORS TUMORIGENICITY
DOI	10.1093/carcin/bgp232
英文摘要	Hepatocellular carcinoma (HCC) is associated with a high morbidity and mortality due to its high rate of recurrence. However, little is known about the biological characteristics of recurrent HCC cells. A single patient's primary and recurrent HCC-derived cell lines, Hep-11 and Hep-12, respectively, were established by primary culture. These two cell lines have the same hepatitis B virus integration site and share many common amplifications and deletions, which suggest that they have the same clonal origin. While Hep-11 cells were non-tumorigenic at 16 weeks following injection of up to 10 000 cells, injection of only 100 Hep-12 cells was sufficient to initiate tumor growth, and all single Hep-12 clones were tumorigenic in immunodeficient mice. Compared with Hep-11, Hep-12 cells expressed the oval cell markers AFP, NCAM/CD56, c-kit/CD117, as well as multiple stem cell markers such as Nanog, OCT4 and SOX2. In addition, > 90% of Hep-12 cells were aldehyde dehydrogenase positive. They were also less resistant to paclitaxel, but more resistant to doxorubicin, cisplatin and hydroxycamptothecin (HCPT), which had been administrated to the patient. Furthermore, Hep-12 cells expressed higher levels of poly (adenosine diphosphate-ribose) polymerase-1 (PARP-1) than Hep-11, and PARP-1 inhibition potentiated the sensitivity to HCPT in Hep-12 cells but not in Hep-11 cells. These results indicate that a large population of the recurrent HCC-derived Hep-12 cells were tumor-initiating cells and that elevated expression of PARP-1 was related to their resistance to HCPT.; Oncology; SCI(E); PubMed; 30; ARTICLE; 2; 167-174; 31
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/344732]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Xiao-Lan,Xing, Bao-Cai,Han, Hai-Bo,et al. The properties of tumor-initiating cells from a hepatocellular carcinoma patient's primary and recurrent tumor[J]. carcinogenesis,2010.
APA	Xu, Xiao-Lan.,Xing, Bao-Cai.,Han, Hai-Bo.,Zhao, Wei.,Hu, Mei-Hao.,...&Zhang, Zhi-Qian.(2010).The properties of tumor-initiating cells from a hepatocellular carcinoma patient's primary and recurrent tumor.carcinogenesis.
MLA	Xu, Xiao-Lan,et al."The properties of tumor-initiating cells from a hepatocellular carcinoma patient's primary and recurrent tumor".carcinogenesis (2010).